Cloning, Annotation and Expression Analysis of Mycoparasitism-Related Genes in Trichoderma harzianum 88

Lin Yao; Qian Yang; Jinzhu Song; Chong Tan; Changhong Guo; Li Wang; Lianhai Qu; Yun Wang

doi:10.1007/s12275-013-2545-7

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Research Support, Non-U.S. Gov't
Cloning, Annotation and Expression Analysis of Mycoparasitism-Related Genes in Trichoderma harzianum 88: Lin Yao ^1,2, Qian Yang ¹, Jinzhu Song ¹, Chong Tan ², Changhong Guo ³, Li Wang ^2,3, Lianhai Qu ¹, Yun Wang ¹; Journal of Microbiology 2013;51(2):174-182
DOI: https://doi.org/10.1007/s12275-013-2545-7
Published online: April 27, 2013

¹Department of Life Science and Engineering, Institute of Technology, Harbin 150001, P. R. China, ²School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, P. R. China, ³Key Laboratory of Molecular and Cytogenetics, Heilongjiang Province, Department of Biology, Harbin Normal University, Harbin 150025, P. R. China¹Department of Life Science and Engineering, Institute of Technology, Harbin 150001, P. R. China, ²School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, P. R. China, ³Key Laboratory of Molecular and Cytogenetics, Heilongjiang Province, Department of Biology, Harbin Normal University, Harbin 150025, P. R. China

Received: 8 October 2012 • Accepted: 22 November 2012

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Abstract

Trichoderma harzianum 88, a filamentous soil fungus, is an effective biocontrol agent against several plant pathogens. High-throughput sequencing was used here to study the mycoparasitism mechanisms of T. harzianum 88. Plate confrontation tests of T. harzianum 88 against plant pathogens were conducted, and a cDNA library was constructed from T. harzianum 88 mycelia in the presence of plant pathogen cell walls. Randomly selected transcripts from the cDNA library were compared with eukaryotic plant and fungal genomes. Of the 1,386 transcripts sequenced, the most abundant Gene Ontology (GO) classification group was “physiological process”. Differential expression of 19 genes was confirmed by real-time RT-PCR at different mycoparasitism stages against plant pathogens. Gene expression analysis revealed the transcription of various genes involved in mycoparasitism of T. harzianum 88. Our study provides helpful insights into the mechanisms of T. harzianum 88-plant pathogen interactions.

Keywords: Trichoderma harzianum 88; cDNA library; expressed sequence tags; plant pathogens; real-time RT-PCR; gene ontology

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Cloning, Annotation and Expression Analysis of Mycoparasitism-Related Genes in Trichoderma harzianum 88

J. Microbiol. 2013;51(2):174-182. Published online April 27, 2013

DOI: https://doi.org/10.1007/s12275-013-2545-7

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