Candida albicans ENO1 Null Mutants Exhibit Altered Drug Susceptibility, Hyphal Formation, and Virulence

Hui-Ching Ko; Ting-Yin Hsiao; Chiung-Tong Chen; Yun-Liang Yang

doi:10.1007/s12275-013-2577-z

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Research Support, Non-U.S. Gov't
Candida albicans ENO1 Null Mutants Exhibit Altered Drug Susceptibility, Hyphal Formation, and Virulence: Hui-Ching Ko ¹, Ting-Yin Hsiao ², Chiung-Tong Chen ³, Yun-Liang Yang ^1,2; Journal of Microbiology 2013;51(3):345-351
DOI: https://doi.org/10.1007/s12275-013-2577-z
Published online: June 28, 2013

¹Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, 300, Taiwan, ²Institute for Molecular Medicine and Bioengineering, National Chiao Tung University, Hsinchu, 300, Taiwan, ³Institute of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Zhunan, Miaoli, 350, Taiwan¹Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, 300, Taiwan, ²Institute for Molecular Medicine and Bioengineering, National Chiao Tung University, Hsinchu, 300, Taiwan, ³Institute of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Zhunan, Miaoli, 350, Taiwan

Corresponding author: Yun-Liang Yang , Tel: +886-3-571-2121,

Received: 22 October 2012 • Accepted: 16 February 2013

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Abstract

We previously showed that the expression of ENO1 (enolase) in the fungal pathogen Candida albicans is critical for cell growth. In this study, we investigate the contribution of the ENO1 gene to virulence. We conducted our functional study of ENO1 in C. albicans by constructing an eno1/eno1 null mutant strain in which both ENO1 alleles in the genome were knockouted with the SAT1 flipper cassette that contains the nourseothricin-resistance marker. Although the null mutant failed to grow on synthetic media containing glucose, it was capable of growth on media containing yeast extract, peptone, and non-fermentable carbon sources. The null mutant was more susceptible to certain antifungal drugs. It also exhibited defective hyphal formation, and was avirulent in BALB/c mice.

Keywords: enolase; opportunistic human pathogen; drug susceptibility; hyphal formation; virulence

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Candida albicans ENO1 Null Mutants Exhibit Altered Drug Susceptibility, Hyphal Formation, and Virulence

J. Microbiol. 2013;51(3):345-351. Published online June 28, 2013

DOI: https://doi.org/10.1007/s12275-013-2577-z

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