Phenotypic and Genotypic Analysis of Clarithromycin-Resistant Helicobacter pylori from Bogotá D.C., Colombia

Alba A. Trespalacios; William Otero; Jorge E. Caminos; Marcela M. Mercado; Jenny Ávila; Liliana E. Rosero; Azucena Arévalo; Raúl A. Poutou-Piñales; David Y. Graham

doi:10.1007/s12275-013-2465-6

Articles

Page Path: HOME > J. Microbiol > Volume 51(4); 2013 > Article

Research Support, Non-U.S. Gov't
Phenotypic and Genotypic Analysis of Clarithromycin-Resistant Helicobacter pylori from Bogotá D.C., Colombia: Alba A. Trespalacios ¹, William Otero ², Jorge E. Caminos ³, Marcela M. Mercado ¹, Jenny Ávila ¹, Liliana E. Rosero ¹, Azucena Arévalo ¹, Raúl A. Poutou-Piñales ⁴, David Y. Graham ^5,6; Journal of Microbiology 2013;51(4):448-452
DOI: https://doi.org/10.1007/s12275-013-2465-6
Published online: August 30, 2013

¹Infectious Diseases Group, Microbiology Department, Science Faculty, Pontificia Universidad Javeriana, Bogotá, D.C., Colombia, ²Gastroenterology Unit. Clínica Fundadores, Bogotá, D.C., - Colombia and Universidad Nacional de Colombia, Bogotá, D.C., - Colombia, ³Biochemistry Unit, Universidad Nacional de Colombia, Bogotá, D.C., Colombia, ⁴Molecular Biotechnology Laboratory, Environment and Industrial Biotechnology Group (GBAI), Microbiology Department, Science Faculty, Pontificia Universidad Javeriana, Bogotá, D.C., Colombia, ⁵Department of Medicine, Michael E. DeBakey Veterans Affairs Medical Center Houston, TX, USA, ⁶Baylor College of Medicine, Houston, TX, USA¹Infectious Diseases Group, Microbiology Department, Science Faculty, Pontificia Universidad Javeriana, Bogotá, D.C., Colombia, ²Gastroenterology Unit. Clínica Fundadores, Bogotá, D.C., - Colombia and Universidad Nacional de Colombia, Bogotá, D.C., - Colombia, ³Biochemistry Unit, Universidad Nacional de Colombia, Bogotá, D.C., Colombia, ⁴Molecular Biotechnology Laboratory, Environment and Industrial Biotechnology Group (GBAI), Microbiology Department, Science Faculty, Pontificia Universidad Javeriana, Bogotá, D.C., Colombia, ⁵Department of Medicine, Michael E. DeBakey Veterans Affairs Medical Center Houston, TX, USA, ⁶Baylor College of Medicine, Houston, TX, USA

Corresponding author: Alba A. Trespalacios , Tel: 571+3208320 ext 4155,

Received: 31 August 2012 • Accepted: 20 February 2013

prev next

3 Views
0 Download
0 Crossref
22 Scopus

Full Article

Abstract

Resistance of Helicobacter pylori to clarithromycin is the most common cause of treatment failure in patients with H. pylori infections. This study describes the MICs and the presence of 23S rRNA mutations of H. pylori isolates from Bogotá, D.C., Colombia. H. pylori were isolated from gastric biopsies from patients with functional dyspepsia. Clarithromycin susceptibility was investigated by agar dilution and strains were considered resistant if the MIC was ≥1 μg/ml. DNA sequences of the 23S rRNA gene of strains resistant and sensitive to clarithromycin were determined to identify specific point mutations. Clarithromycin resistance was present in 13.6% of patients by agar dilution. The A2143G, A2142G and A2142C mutations were found in 90.5, 7.1, and 2.4% of H. pylori strains with resistance genotype.The resistant phenotype was associated with 23S rRNA resistance genotype in 85.7% of isolates. The point mutations in 23S rRNA were well correlated with MICs values for clarithromycin.

Keywords: Helicobacter pylori; clarithromycin resistance; 23S rRNA mutations

Figure & Data

References

Citations

Citations to this article as recorded by

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Phenotypic and Genotypic Analysis of Clarithromycin-Resistant Helicobacter pylori from Bogotá D.C., Colombia

J. Microbiol. 2013;51(4):448-452. Published online August 30, 2013

DOI: https://doi.org/10.1007/s12275-013-2465-6

Related articles