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HOME > J. Microbiol > Volume 51(6); 2013 > Article
Research Support, Non-U.S. Gov't
Evaluation of Bakanae Disease Progression Caused by Fusarium fujikuroi in Oryza sativa L.
In Sun Hwang 1, Woo-Ri Kang 1, Duk-Ju Hwang 1, Shin-Chul Bae 1, Sung-Hwan Yun 2, Il-Pyung Ahn 1
Journal of Microbiology 2013;51(6):858-865.
DOI: https://doi.org/10.1007/s12275-013-3472-3
Published online: December 19, 2013
1National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea, 2Department of Medical Biotechnology, Soonchunhyang University, Asan 336-745, Republic of Korea1National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea, 2Department of Medical Biotechnology, Soonchunhyang University, Asan 336-745, Republic of Korea
Corresponding author:  Il-Pyung Ahn , Tel: +82-31-299-1724, 
Received: 9 September 2013   • Accepted: 7 October 2013
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Abstract

Bakanae disease caused by Fusarium fujikuroi is an important fungal disease in rice. Among the seven strains isolated from symptomatic rice grains in this study, one strain, FfB14, triggered severe root growth inhibition and decay in the crown and root of rice seedlings. The remaining six strains caused typical Bakanae symptoms such as etiolation and abnormal succulent rice growth. To reveal the relationship between mycelial growth in the infected tissues and Bakanae disease progression, we have established a reliable quantification
method
using real time PCR that employs a primer pair and dual-labeled probe specific to a unigene encoding F. fujikuroi PNG1 (FfPNG1), which is located upstream of the fumonisin biosynthesis gene cluster. Plotting the crossing point (CP) values from the infected tissue DNAs on a standard curve revealed the active fungal growth of FfB14 in the root and crown of rice seedlings, while the growth rate of FfB20 in rice was more than 4 times lower than FfB14. Massive infective mycelial growth of FfB14 was evident in rice stems and crown; however, FfB20 did not exhibit vigorous growth. Our quantitative evaluation system is applicable for the identification of fungal virulence factors other than gibberellin.

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    Evaluation of Bakanae Disease Progression Caused by Fusarium fujikuroi in Oryza sativa L.
    J. Microbiol. 2013;51(6):858-865.   Published online December 19, 2013
    DOI: https://doi.org/10.1007/s12275-013-3472-3
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