Abstract
Antifungal lipopeptide produced by Bacillus sp. BH072 was extracted from fermentation liquor and determined as iturin A by liquid chromatography-mass spectrometry (LC-MS). For industrial-scale production, the yield of iturin A was
improved by optimizing medium components and fermentation conditions. A one-factor test was conducted; fermentation conditions were then optimized by response surface methodology (RSM) to obtain the following: temperature, 29.5°C; pH 6.45; inoculation quantity, 6.7%; loading volume, 100 ml (in 500 ml flasks); and rotary speed, 150 rpm. Under these conditions, the mass concentration of iturin A was increased from 45.30 mg/ml to 47.87 mg/ml. The following components of the medium were determined: carbon sources (glucose, fructose, sucrose, xylose, rhamnose, and soluble starch); nitrogen sources (peptone, soybean meal, NH4Cl, urea, and ammonium citrate); and metal ions (Zn2+, Fe3+, Mg2+, Mn2+, Ca2+, and K+). The effects of these components on iturin A production were observed in LB medium. We
selected sucrose, soybean meal, and Mg2+ for RSM to optimize the conditions because of several advantages, including maximum iturin A production, high antifungal activity, and low cost. The optimum concentrations of these components
were 0.98% sucrose, 0.94% soybean meal, and 0.93% Mg2+. After iturin A production was optimized by RSM, the mass concentration reached 52.21 mg/ml. The antifungal specific activity was enhanced from 350.11 AU/mg to 513.92
AU/mg, which was 46.8% higher than the previous result. The present study provides an important experimental basis for the industrial-scale production of iturin A and the agricultural applications of Bacillus sp. BH072.
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- Medium Optimization and Fermentation Kinetics for Antifungal Compounds Production by an Endophytic Paenibacillus polymyxa DS-R5 Isolated from Salvia miltiorrhiza
Rongbo Sa, Yue Sun, Ying Cao, Wenhui Yan, Zhaohui Zong, Wen An, Meimei Song
Current Microbiology.2024;[Epub] CrossRef - Optimization of concentration parameters for maximizing bacteriophage recovery value using response surface methodology: A case study on determining the optimal concentration parameters for Erwinia amylovoraEA1T1.B3 phage
Kubra Guven, Esra Ekiz, Eylul Evran, Emine Kubra Tayyarcan, Ismail Hakki Boyaci
Plant Pathology.2024; 73(5): 1090. CrossRef - Characterization of differences in seed endophytic microbiome in conventional and organic rice by amplicon-based sequencing and culturing methods
Sabin Khanal, Muhammad Imran, Xin-Gen Zhou, Sanjay Antony-Babu, Se-Ran Jun
Microbiology Spectrum.2024;[Epub] CrossRef - Antimicrobial peptides from Bacillus spp. and strategies to enhance their yield
Sheau Ling Puan, Pirasannah Erriah, Mohamad Malik Al-adil Baharudin, Normi Mohd Yahaya, Wan Nur Ismah Wan Ahmad Kamil, Mohd Shukuri Mohamad Ali, Siti Aqlima Ahmad, Siti Nurbaya Oslan, Sooa Lim, Suriana Sabri
Applied Microbiology and Biotechnology.2023; 107(18): 5569. CrossRef - Optimization of the Fermentation Conditions of Metarhizium robertsii and Its Biological Control of Wolfberry Root Rot Disease
Jing He, Xiaoyan Zhang, Qinghua Wang, Nan Li, Dedong Ding, Bin Wang
Microorganisms.2023; 11(10): 2380. CrossRef - Optimization of the Production and Characterization of an Antifungal Protein by Bacillus velezensis Strain NT35 and Its Antifungal Activity against Ilyonectria robusta Causing Ginseng Rusty Root Rot
Mengtao Li, Hao Tang, Zongyan Li, Yu Song, Lin Chen, Chao Ran, Yun Jiang, Changqing Chen
Fermentation.2023; 9(4): 358. CrossRef - Isolation and Genome-Based Characterization of Biocontrol Potential of Bacillus siamensis YB-1631 against Wheat Crown Rot Caused by Fusarium pseudograminearum
Qianqian Dong, Qingxiang Liu, Paul H. Goodwin, Xiaoxu Deng, Wen Xu, Mingcong Xia, Jie Zhang, Runhong Sun, Chao Wu, Qi Wang, Kun Wu, Lirong Yang
Journal of Fungi.2023; 9(5): 547. CrossRef - Enhanced bacillomycin D yield by cyclic fermentation with diatomaceous earth immobilized Bacillus amyloliquefaciens fmbJ
Xiaojiao Luo, Jin Zhang, Yongjin Dai, Zhaoxin Lu, Jing Sun, Yingjian Lu
Process Biochemistry.2023; 132: 278. CrossRef - Assessment of Lipopeptide Mixtures Produced by Bacillus subtilis as Biocontrol Products against Apple Scab (Venturia inaequalis)
Aline Leconte, Ludovic Tournant, Jérôme Muchembled, Jonathan Paucellier, Arnaud Héquet, Barbara Deracinois, Caroline Deweer, François Krier, Magali Deleu, Sandrine Oste, Philippe Jacques, François Coutte
Microorganisms.2022; 10(9): 1810. CrossRef - Promoted Spore Formation of Bacillus amyloliquefaciens fmbJ by its Secondary Metabolite Bacillomycin D Coordinated with Mn2+
Jin Zhang, Xiaojiao Luo, Xinyi Pang, Xiangfei Li, Yingjian Lu, Jing Sun
Indian Journal of Microbiology.2022; 62(4): 531. CrossRef - Optimization of Bacillus amyloliquefaciens BLB369 Culture Medium by Response Surface Methodology for Low Cost Production of Antifungal Activity
Imen Zalila-Kolsi, Sameh Kessentini, Slim Tounsi, Kaïs Jamoussi
Microorganisms.2022; 10(4): 830. CrossRef - Lipopeptide Biosurfactants from Bacillus spp.: Types, Production, Biological Activities, and Applications in Food
Nawazish Ali, Zhengjun Pang, Fenghuan Wang, Baocai Xu, Hesham R. El-Seedi, Abid Hussain
Journal of Food Quality.2022; 2022: 1. CrossRef - Production of volatile compounds and lipopeptides as antagonistic mechanisms of two Bacillus strains towards phytopathogenic fungi
Javier Ramírez-Martínez, Juan Ramiro Pacheco-Aguilar
Revista de Simulación y Laboratorio.2022; : 29. CrossRef - Isolation and identification of a new biocontrol bacteria against Salvia miltiorrhiza root rot and optimization of culture conditions for antifungal substance production using response surface methodology
Rongbo Sa, Song He, Dongdong Han, Mengjiao Liu, Yunxia Yu, Rongen Shang, Meimei Song
BMC Microbiology.2022;[Epub] CrossRef - Statistical optimization of exopolysaccharide production by Leuconostoc pseudomesenteroides JF17 from native Atlantic Forest juçara fruit
Fernanda Silva Farinazzo, Maria Thereza Carlos Fernandes, Carolina Saori Ishii Mauro, Sandra Garcia
Preparative Biochemistry & Biotechnology.2022; 52(3): 245. CrossRef - Isolation, Characterisation and Fermentation Optimisation of Bacteriocin-Producing Enterococcus faecium
Xiaoxiao Qiao, Renpeng Du, Yu Wang, Ye Han, Zhijiang Zhou
Waste and Biomass Valorization.2020; 11(7): 3173. CrossRef - Bacillus velezensis 83 a bacterial strain from mango phyllosphere, useful for biological control and plant growth promotion
Karina A. Balderas-Ruíz, Patricia Bustos, Rosa I. Santamaria, Víctor González, Sergio Andrés Cristiano-Fajardo, Salvador Barrera-Ortíz, Miriam Mezo-Villalobos, Sergio Aranda-Ocampo, Ángel Arturo Guevara-García, Enrique Galindo, Leobardo Serrano-Carreón
AMB Express.2020;[Epub] CrossRef - The study of the antifungal activity of the Bacillus subtilis BZR 336g strain under the conditions of periodic cultivation with the addition of citric acid, corn extract and some microelements
Anzhela Asaturova, Evgeny Gyrnets, Valeria Allakhverdian, Mikhail Astakhov, Ksenia Saenko, A. Asaturova, E. Esaulenko
BIO Web of Conferences.2020; 21: 00015. CrossRef - Identification and characterization of the Bacillus atrophaeus strain J-1 as biological agent of apple ring rot disease
Yangping Mu, Ying Yue, Guirong Gu, Yiming Deng, Hong Jin, Ke Tao
Journal of Plant Diseases and Protection.2020; 127(3): 367. CrossRef - Enhanced production of antifungal lipopeptide iturin A by Bacillus amyloliquefaciens LL3 through metabolic engineering and culture conditions optimization
Yulei Dang, Fengjie Zhao, Xiangsheng Liu, Xu Fan, Rui Huang, Weixia Gao, Shufang Wang, Chao Yang
Microbial Cell Factories.2019;[Epub] CrossRef - Biological control of plant pathogens by Bacillus species
Djordje Fira, Ivica Dimkić, Tanja Berić, Jelena Lozo, Slaviša Stanković
Journal of Biotechnology.2018; 285: 44. CrossRef - Enhanced production of exopolysaccharides using industrial grade starch as sole carbon source
Xun He, Feng He, Jiao Hang, Hui Li, Yali Chen, Ping Wei, Kequan Chen, Yan Li, Pingkai OuYang
Bioprocess and Biosystems Engineering.2018; 41(6): 811. CrossRef - Optimization, chain conformation and characterization of exopolysaccharide isolated from Leuconostoc mesenteroides DRP105
Hanwen Xing, Renpeng Du, Fangkun Zhao, Ye Han, Huazhi Xiao, Zhijiang Zhou
International Journal of Biological Macromolecules.2018; 112: 1208. CrossRef - High-throughput optimization of medium components and culture conditions for the efficient production of a lipopeptide pseudofactin by Pseudomonas fluorescens BD5
Piotr Biniarz, François Coutte, Frédérique Gancel, Marcin Łukaszewicz
Microbial Cell Factories.2018;[Epub] CrossRef - Differential antagonistic responses of Bacillus pumilus MSUA3 against Rhizoctonia solani and Fusarium oxysporum causing fungal diseases in Fagopyrum esculentum Moench
Mohit Agarwal, Shrivardhan Dheeman, Ramesh Chand Dubey, Pradeep Kumar, Dinesh Kumar Maheshwari, Vivek K. Bajpai
Microbiological Research.2017; 205: 40. CrossRef - Improvement of biomass and cyclic lipopeptides production in Bacillus amyloliquefaciens MEP218 by modifying carbon and nitrogen sources and ratios of the culture media
Daniela B. Medeot, Magdalena Bertorello-Cuenca, Juan Pablo Liaudat, Florencia Alvarez, María Laura Flores-Cáceres, Edgardo Jofré
Biological Control.2017; 115: 119. CrossRef - Antifungal Effects of Lipopeptide Produced by <i>Bacillus amyloliquefaciens</i> BH072
Xin Zhao, Zhijiang Zhou, Ye Han
Advances in Bioscience and Biotechnology.2017; 08(09): 295. CrossRef - Optimization, purification and structural characterization of a dextran produced by L. mesenteroides isolated from Chinese sauerkraut
Renpeng Du, Hanwen Xing, Yanping Yang, Hanji Jiang, Zhijiang Zhou, Ye Han
Carbohydrate Polymers.2017; 174: 409. CrossRef - Extracts containing CLPs of Bacillus amyloliquefaciens JN68 isolated from chicken intestines exert antimicrobial effects, particularly on methicillin-resistant Staphylococcus aureus and Listeria monocytogenes
Jen-Ni Chen, Chyou-Wei Wei, Hsiao-Chun Liu, Shu-Ying Chen, Chinshuh Chen, Yu-Min Juang, Chien-Chen Lai, Giou-Teng Yiang
Molecular Medicine Reports.2016; 14(6): 5155. CrossRef - Optimization of the Expression Conditions of CGA-N46 in Bacillus subtilis DB1342(p-3N46) by Response Surface Methodology
Rui-Fang Li, Bin Wang, Shuai Liu, Shi-Hua Chen, Guang-Hai Yu, Shuo-Ye Yang, Liang Huang, Yan-Li Yin, Zhi-Fang Lu
Interdisciplinary Sciences: Computational Life Sciences.2016; 8(3): 277. CrossRef - Identification and classification of known and putative antimicrobial compounds produced by a wide variety of Bacillales species
Xin Zhao, Oscar P. Kuipers
BMC Genomics.2016;[Epub] CrossRef - Review lipopeptides biosurfactants: Mean classes and new insights for industrial, biomedical, and environmental applications
Inès Mnif, Dhouha Ghribi
Peptide Science.2015; 104(3): 129. CrossRef - Continuous enhancement of iturin A production by Bacillus subtilis with a stepwise two-stage glucose feeding strategy
Hu Jin, Kunpeng Li, Yanxing Niu, Mian Guo, Chuanjiong Hu, Shouwen Chen, Fenghong Huang
BMC Biotechnology.2015;[Epub] CrossRef - Lipopeptide surfactants: Production, recovery and pore forming capacity
Mnif Inès, Ghribi Dhouha
Peptides.2015; 71: 100. CrossRef - Identification and characterization of the endophytic bacterium Bacillus atrophaeus XW2, antagonistic towards Colletotrichum gloeosporioides
Huayi Huang, Ziqiang Wu, Chengming Tian, Yingmei Liang, Chongjuan You, Lei Chen
Annals of Microbiology.2015; 65(3): 1361. CrossRef