A Potent Brucella abortus 2308 Δery Live Vaccine Allows for the Differentiation between Natural and Vaccinated Infection

Junbo Zhang; Shuanghong Yin; Fei Guo; Ren Meng; Chuangfu Chen; Hui Zhang; Zhiqiang Li; Qiang Fu; Huijun Shi; Shengwei Hu; Wei Ni; Tiansen Li; Ke Zhang

doi:10.1007/s12275-014-3689-9

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Research Support, Non-U.S. Gov't
A Potent Brucella abortus 2308 Δery Live Vaccine Allows for the Differentiation between Natural and Vaccinated Infection: Junbo Zhang ¹, Shuanghong Yin ², Fei Guo ², Ren Meng ¹, Chuangfu Chen ¹, Hui Zhang ¹, Zhiqiang Li ¹, Qiang Fu ¹, Huijun Shi ¹, Shengwei Hu ¹, Wei Ni ³, Tiansen Li ¹, Ke Zhang ¹; Journal of Microbiology 2014;52(8):681-688
DOI: https://doi.org/10.1007/s12275-014-3689-9
Published online: July 4, 2014

¹College of Animal Science and Technology, Shihezi University, Shihezi 832003, P. R. China, ²College of Medicine, Shihezi University, Shihezi 832003, P. R. China, ³College of Life Sciences, Shihezi University, Xinjiang 832000, P. R. China¹College of Animal Science and Technology, Shihezi University, Shihezi 832003, P. R. China, ²College of Medicine, Shihezi University, Shihezi 832003, P. R. China, ³College of Life Sciences, Shihezi University, Xinjiang 832000, P. R. China

Corresponding author: Chuangfu Chen , Tel: +86-0993-2058002,

Received: 30 December 2013 • Revised: 24 April 2014 • Accepted: 1 May 2014

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Abstract

Brucellosis is a globally distributed zoonotic disease that causes animal and human diseases. However, the current Brucella abortus vaccines (S19 and RB51) are deficient; they can cause abortion in pregnant animals. Moreover, when the vaccine S19 is used, tests cannot differentiate natural from vaccinated infection. Therefore, a safer and more potent vaccine is needed. A Brucella abortus 2308 ery promoter mutant (Δery) was constructed to overcome these drawbacks. The growth of the Δery mutant was significantly attenuated in macrophages and mice and induced high protective immunity in mice. Moreover, Δery induced an anti-Brucellaspecific IgG (immunoglobulin G) response and stimulated the expression of interferon-gamma (INF-γ) and interleukin-4 (IL-4). Furthermore, the expression of EryA antigen allowed for the serological differentiation between natural and vaccinated infection in mice. These results indicate that the Δery mutant is a potential attenuated live vaccine candidate against virulent Brucella abortus 2308 (S2308) infection.

Keywords: Brucella abortus 2308; ery promoter mutant; live attenuated vaccine; EryA

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A Potent Brucella abortus 2308 Δery Live Vaccine Allows for the Differentiation between Natural and Vaccinated Infection

J. Microbiol. 2014;52(8):681-688. Published online July 4, 2014

DOI: https://doi.org/10.1007/s12275-014-3689-9

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