A Potent Brucella abortus 2308 Δery Live Vaccine Allows for the Differentiation between Natural and Vaccinated Infection

Junbo Zhang; Shuanghong Yin; Fei Guo; Ren Meng; Chuangfu Chen; Hui Zhang; Zhiqiang Li; Qiang Fu; Huijun Shi; Shengwei Hu; Wei Ni; Tiansen Li; Ke Zhang

doi:10.1007/s12275-014-3689-9

Articles

Page Path: HOME > J. Microbiol > Volume 52(8); 2014 > Article

Research Support, Non-U.S. Gov't
A Potent Brucella abortus 2308 Δery Live Vaccine Allows for the Differentiation between Natural and Vaccinated Infection: Junbo Zhang ¹, Shuanghong Yin ², Fei Guo ², Ren Meng ¹, Chuangfu Chen ¹, Hui Zhang ¹, Zhiqiang Li ¹, Qiang Fu ¹, Huijun Shi ¹, Shengwei Hu ¹, Wei Ni ³, Tiansen Li ¹, Ke Zhang ¹; Journal of Microbiology 2014;52(8):681-688
DOI: https://doi.org/10.1007/s12275-014-3689-9
Published online: July 4, 2014

¹College of Animal Science and Technology, Shihezi University, Shihezi 832003, P. R. China, ²College of Medicine, Shihezi University, Shihezi 832003, P. R. China, ³College of Life Sciences, Shihezi University, Xinjiang 832000, P. R. China¹College of Animal Science and Technology, Shihezi University, Shihezi 832003, P. R. China, ²College of Medicine, Shihezi University, Shihezi 832003, P. R. China, ³College of Life Sciences, Shihezi University, Xinjiang 832000, P. R. China

Corresponding author: Chuangfu Chen , Tel: +86-0993-2058002,

Received: 30 December 2013 • Revised: 24 April 2014 • Accepted: 1 May 2014

prev next

14 Views
0 Download
0 Crossref
16 Scopus

Full Article

Abstract

Brucellosis is a globally distributed zoonotic disease that causes animal and human diseases. However, the current Brucella abortus vaccines (S19 and RB51) are deficient; they can cause abortion in pregnant animals. Moreover, when the vaccine S19 is used, tests cannot differentiate natural from vaccinated infection. Therefore, a safer and more potent vaccine is needed. A Brucella abortus 2308 ery promoter mutant (Δery) was constructed to overcome these drawbacks. The growth of the Δery mutant was significantly attenuated in macrophages and mice and induced high protective immunity in mice. Moreover, Δery induced an anti-Brucellaspecific IgG (immunoglobulin G) response and stimulated the expression of interferon-gamma (INF-γ) and interleukin-4 (IL-4). Furthermore, the expression of EryA antigen allowed for the serological differentiation between natural and vaccinated infection in mice. These results indicate that the Δery mutant is a potential attenuated live vaccine candidate against virulent Brucella abortus 2308 (S2308) infection.

Keywords: Brucella abortus 2308; ery promoter mutant; live attenuated vaccine; EryA

Figure & Data

References

Citations

Citations to this article as recorded by

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

A Potent Brucella abortus 2308 Δery Live Vaccine Allows for the Differentiation between Natural and Vaccinated Infection

J. Microbiol. 2014;52(8):681-688. Published online July 4, 2014

DOI: https://doi.org/10.1007/s12275-014-3689-9

Related articles