Skip Navigation
Skip to contents

Journal of Microbiology : Journal of Microbiology

OPEN ACCESS
SEARCH
Search
Advanced Search
mobile menu button

Articles

Page Path
HOME > J. Microbiol > Volume 52(8); 2014 > Article
Research Support, Non-U.S. Gov't
Evaluation of the Antimicrobial Potency of Silver Nanoparticles Biosynthesized by Using an Endophytic Fungus, Cryptosporiopsis ericae PS4
Lamabam Sophiya Devi , Santa Ram Joshi
Journal of Microbiology 2014;52(8):667-674.
DOI: https://doi.org/10.1007/s12275-014-4113-1
Published online: July 4, 2014
Microbiology Laboratory, Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong 793022, IndiaMicrobiology Laboratory, Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong 793022, India
Corresponding author:  Santa Ram Joshi , Tel: +919436102171, 
Received: 21 February 2014   • Revised: 17 April 2014   • Accepted: 21 April 2014
prev next
  • 63 Views
  • 0 Download
  • 34 Crossref
  • 56 Scopus
Full Article

Abstract

In the present study, silver nanoparticles (AgNPs) with an average particle size of 5.5 ± 3.1 nm were biosynthesized using an endophytic fungus Cryptosporiopsis ericae PS4 isolated from the ethno-medicinal plant Potentilla fulgens L. The nanoparticles were characterized using UV-visible spectrophotometer, transmission electron microscopy (TEM), scanning electron microscopy (SEM), selective area electron diffraction (SAED), and energy dispersive X-ray (EDX) spectroscopy analysis. Antimicrobial efficacy of the AgNPs was analyzed singly and in combination with the antibiotic/antifungal agent chloramphenicol/fluconazole, against five pathogenic microorganisms-Staphylococcus aureus MTCC96, Salmonella enteric MTCC735, Escherichia coli MTCC730, Enterococcus faecalis MTCC2729, and Candida albicans MTCC 183. The activity of AgNPs on the growth and morphology of the microorganisms was studied in solid and liquid growth media employing various susceptibility assays. These studies demonstrated that concentrations of AgNPs alone between 10 and 25 μM reduced the growth rates of the tested bacteria and fungus and revealed bactericidal/fungicidal activity of the AgNPs by delaying the exponential and stationary phases. Examination using SEM showed pits and ruptures in bacterial cells indicating fragmented cell membrane and severe cell damage in those cultures treated with AgNPs. These experimental findings suggest that the biosynthesized AgNPs may be a potential antimicrobial agent.

  • Cite this Article
    Cite this Article
    export Copy Download
    Close
    Download Citation
    Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.
    Format:
    • RIS — For EndNote, ProCite, RefWorks, and most other reference management software
    • BibTeX — For JabRef, BibDesk, and other BibTeX-specific software
    Include:
    • Citation for the content below
    Evaluation of the Antimicrobial Potency of Silver Nanoparticles Biosynthesized by Using an Endophytic Fungus, Cryptosporiopsis ericae PS4
    J. Microbiol. 2014;52(8):667-674.   Published online July 4, 2014
    DOI: https://doi.org/10.1007/s12275-014-4113-1
    Close
Related articles
Journal of Microbiology : Journal of Microbiology
© The Microbiological Society of Korea.
TOP