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Research Support, Non-U.S. Gov't
- NOTE] A Protective Role of Methionine-R-Sulfoxide Reductase against Cadmium in Schizosaccharomyces pombe
- Chang-Jin Lim 1, Hannah Jo 1, Kyunghoon Kim 2
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Journal of Microbiology 2014;52(11):976-981
DOI: https://doi.org/10.1007/s12275-014-3512-7
Published online: May 30, 2014
1Department of Biochemistry, Kangwon National University, Chuncheon 200-701, Republic of Korea, 2Department of Biological Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea1Department of Biochemistry, Kangwon National University, Chuncheon 200-701, Republic of Korea, 2Department of Biological Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea
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Corresponding author:
Kyunghoon Kim , Tel: +82-33-250-8527,
Received: 2 October 2013 • Revised: 7 January 2014 • Accepted: 9 January 2014
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Abstract
The Schizosaccharomyces pombe cells harboring the methionine- R-sulfoxide reductase (MsrB)-overexpressing recombinant plasmid pFMetSO exhibited better growth than vector control cells, when shifted into fresh medium containing cadmium chloride (abbreviated as Cd). Although both groups of cells contained enhanced reactive oxygen species (ROS) and nitric oxide (NO) levels in the presence of Cd, ROS and NO levels were significantly lower in the S. pombe cells harboring pFMetSO than in vector control cells. Conversely, the S. pombe cells harboring pFMetSO possessed higher total glutathione (GSH) levels and a greater reduced/oxidized GSH ratio than vector control cells under the same conditions.
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