Note] Analysis of a draft genome sequence of Kitasatospora cheerisanensis KCTC 2395 producing bafilomycin antibiotics

Jae Yoon Hwang; Soo Hee Kim; Hye Ryeung Oh; Eunju Kwon; Doo Hyun Nam

doi:10.1007/s12275-015-4340-0

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Research Support, Non-U.S. Gov't
Note] Analysis of a draft genome sequence of Kitasatospora cheerisanensis KCTC 2395 producing bafilomycin antibiotics: Jae Yoon Hwang ¹, Soo Hee Kim ¹, Hye Ryeung Oh ¹, Eunju Kwon ², Doo Hyun Nam ¹; Journal of Microbiology 2015;53(1):84-89.
DOI: https://doi.org/10.1007/s12275-015-4340-0
Published online: December 4, 2014

¹College of Pharmacy, Yeungnam University, Gyongsan 712-749, Republic of Korea, ²Institute for Drug Development, Yeungnam University, Gyongsan 712-749, Republic of Korea¹College of Pharmacy, Yeungnam University, Gyongsan 712-749, Republic of Korea, ²Institute for Drug Development, Yeungnam University, Gyongsan 712-749, Republic of Korea

Corresponding author: Doo Hyun Nam , Tel: +82-53-810-2825,

Received: 13 June 2014 • Revised: 31 July 2014 • Accepted: 20 August 2014

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Abstract

Kitasatospora cheerisanensis KCTC 2395, producing bafilomycin antibiotics belonging to plecomacrolide group, was isolated from a soil sample at Mt. Jiri, Korea. The draft genome sequence contains 8.04 Mb with 73.6% G+C content and 7,810 open reading frames. All the genes for aerial mycelium and spore formations were confirmed in this draft genome. In phylogenetic analysis of MurE proteins (UDPN- acetylmuramyl-L-alanyl-D-glutamate:DAP ligase) in a conserved dcw (division of cell wall) locus, MurE proteins of Kitasatospora species were placed in a separate clade between MurEs of Streptomyces species incorporating LL-diaminopimelic acid (DAP) and MurEs of Saccharopolyspora erythraea as well as Mycobacterium tuberculosis ligating meso- DAP. From this finding, it was assumed that Kitasatospora MurEs exhibit the substrate specificity for both LL-DAP and meso-DAP. The bafilomycin biosynthetic gene cluster was located in the left subtelomeric region. In 71.3 kb-long gene cluster, 17 genes probably involved in the biosynthesis of bafilomycin derivatives were deduced, including 5 polyketide synthase (PKS) genes comprised of 12 PKS modules.

Keywords: draft genome;Kitasatospora;MurE;polyketide synthase;bafilomycin

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Note] Analysis of a draft genome sequence of Kitasatospora cheerisanensis KCTC 2395 producing bafilomycin antibiotics

J. Microbiol. 2015;53(1):84-89. Published online December 4, 2014

DOI: https://doi.org/10.1007/s12275-015-4340-0

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