Genetic diversity of Clavispora lusitaniae isolated from Agave fourcroydes Lem, as revealed by DNA fingerprinting

Daisy Pérez-Brito; Anuar Magaña-Alvarez; Patricia Lappe-Oliveras; Alberto Cortes-Velazquez; Claudia Torres-Calzada; Teófilo Herrera-Suarez; Alfonso Larqué-Saavedra; Raul Tapia-Tussell

doi:10.1007/s12275-015-4373-4

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Genetic diversity of Clavispora lusitaniae isolated from Agave fourcroydes Lem, as revealed by DNA fingerprinting: Daisy Pérez-Brito ¹, Anuar Magaña-Alvarez ¹, Patricia Lappe-Oliveras ², Alberto Cortes-Velazquez ¹, Claudia Torres-Calzada ¹, Teófilo Herrera-Suarez ², Alfonso Larqué-Saavedra ³, Raul Tapia-Tussell ¹; Journal of Microbiology 2015;53(1):14-20
DOI: https://doi.org/10.1007/s12275-015-4373-4
Published online: January 4, 2015

¹Laboratorio GeMBio, Centro de Investigación Científica de Yucatán A.C., Calle 43 # 130, Col. Chuburná de Hidalgo, Mérida, Yucatán 97200, México, ²Instituto de Biología, Universidad Nacional Autónoma de México, 3er Circuito S/N, Ciudad Universitaria, Del. Coyoacán, México DF. 04510, México, ³Centro de Investigación Científica de Yucatán A.C., Calle 43 # 130, Col. Chuburná de Hidalgo, Mérida, Yucatán 97200, México¹Laboratorio GeMBio, Centro de Investigación Científica de Yucatán A.C., Calle 43 # 130, Col. Chuburná de Hidalgo, Mérida, Yucatán 97200, México, ²Instituto de Biología, Universidad Nacional Autónoma de México, 3er Circuito S/N, Ciudad Universitaria, Del. Coyoacán, México DF. 04510, México, ³Centro de Investigación Científica de Yucatán A.C., Calle 43 # 130, Col. Chuburná de Hidalgo, Mérida, Yucatán 97200, México

Corresponding author: Raul Tapia-Tussell , Tel: +52-9999428369,

Received: 25 June 2014 • Revised: 24 October 2014 • Accepted: 1 November 2014

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Abstract

This study characterized Clavispora lusitaniae strains isolated from different stages of the processing and early fermentation of a henequen (Agave fourcroydes) spirit produced in Yucatan, Mexico using a molecular technique. Sixteen strains identified based on morphological features, obtained from different substrates, were typed molecularly. Nine different versions of the divergent D1/D2 domain of the large-subunit ribosomal DNA sequence were identified among the C. lusitaniae strains. The greatest degree of polymorphism was found in the 90-bp structural motif of the D2 domain. The MSP-PCR technique was able to differentiate 100% of the isolates. This study provides significant insight into the genetic diversity of the mycobiota present during the henequen fermentation process, especially that of C. lusitaniae, for which only a few studies in plants have been published. The applied MSP-PCR markers were very efficient in revealing polymorphisms between isolates of this species.

Keywords: Agave fourcroydes; Clavispora lusitaniae; D1/D2 large-subunit rDNA polymorphism; DNA fingerprinting; genetic diversity

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Genetic diversity of Clavispora lusitaniae isolated from Agave fourcroydes Lem, as revealed by DNA fingerprinting

J. Microbiol. 2015;53(1):14-20. Published online January 4, 2015

DOI: https://doi.org/10.1007/s12275-015-4373-4

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