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Research Support, Non-U.S. Gov't
- Effect of promoter-upstream sequence on σ38-dependent stationary phase gene transcription
- Hyung-Ju Lim , Kwangsoo Kim , Minsang Shin , Jae-Ho Jeong , Phil Youl Ryu , Hyon E. Choy
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Journal of Microbiology 2015;53(4):250-255
DOI: https://doi.org/10.1007/s12275-015-4681-8
Published online: April 8, 2015
Department of Microbiology, Chonnam National University Medical School, Kwangju 501-746, Republic of KoreaDepartment of Microbiology, Chonnam National University Medical School, Kwangju 501-746, Republic of Korea
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Corresponding author:
Hyon E. Choy , Tel: +82-62-220-4137,
Received: 3 December 2014 • Revised: 15 January 2015 • Accepted: 26 January 2015
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Abstract
σ38 in Escherichia coli is required for expression of a subset
of stationary phase genes. However, the promoter elements
for σ38-dependent genes are virtually indistinguishable from
that for σ70-dependent house-keeping genes. hdeABp is a
σ38-dependent promoter and LEE5p is a σ70-dependent
promoter, but both are repressed by H-NS, a bacterial histone-
like protein, which acts at promoter upstream sequence.
We swapped the promoter upstream sequences of the two
promoters and found that the σ dependency was switched.
This was further verified using lacUV5 core promoter. The
results
suggested that the determinant for σ38-dependent
promoter lies in the promoter upstream sequence.
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