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HOME > J. Microbiol > Volume 53(4); 2015 > Article
Research Support, U.S. Gov't, Non-P.H.S.
Multiple cellular roles of Neurospora crassa plc-1, splA2, and cpe-1 in regulation of cytosolic free calcium, carotenoid accumulation, stress responses, and acquisition of thermotolerance§
Ananya Barman , Ranjan Tamuli
Journal of Microbiology 2015;53(4):226-235.
DOI: https://doi.org/10.1007/s12275-015-4465-1
Published online: January 31, 2015
Department of Biotechnology, Indian Institute of Technology Guwahati Guwahati 781 039, IndiaDepartment of Biotechnology, Indian Institute of Technology Guwahati Guwahati 781 039, India
Corresponding author:  Ranjan Tamuli , Tel: +91-361-258-2220, 
Received: 12 August 2014   • Revised: 12 December 2014   • Accepted: 15 December 2014
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Abstract

Phospholipase C1 (PLC1), secretory phospholipase A2 (sPLA2) and Ca2+/H+ exchanger proteins regulate calcium signaling and homeostasis in eukaryotes. In this study, we investigate functions for phospholipase C1 (plc-1), sPLA2 (splA2) and a Ca2+/H+ exchanger (cpe-1) in the filamentous fungus Neurospora crassa. The Δplc-1, ΔsplA2, and Δcpe-1 mutants exhibited a growth defect on medium supplemented with the divalent ionophore A23187, suggesting that these genes might play a role in regulation of cytosolic free Ca2+ concentration ([Ca2+]c) in N. crassa. The strains lacking plc-1, splA2, and cpe-1 possessed higher carotenoid content than wild type at 8°C, 22°C, and 30°C, and showed increased ultraviolet (UV)- survival under conditions that induced carotenoid accumulation. Moreover, Δplc-1, ΔsplA2, and Δcpe-1 mutants showed reduced survival rate under hydrogen peroxide-induced oxidative stress and induced thermotolerance after exposure to heat shock temperatures. Thus, this study revealed multiple cellular roles for plc-1, splA2, and cpe-1 genes in regulation of [Ca2+]c, carotenoid accumulation, survival under stress conditions, and acquisition of thermotolerance induced by heat shock.

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    Multiple cellular roles of Neurospora crassa plc-1, splA2, and cpe-1 in regulation of cytosolic free calcium, carotenoid accumulation, stress responses, and acquisition of thermotolerance§
    J. Microbiol. 2015;53(4):226-235.   Published online January 31, 2015
    DOI: https://doi.org/10.1007/s12275-015-4465-1
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