Characterization and Identification of the Bacteriophage P4 Mutant Suppressin sir Mutations of Bacteriophage P2

Kim, Kyoung Jin; Sunshine, Melvin G.; Six, Erich W.


Characterization and Identification of the Bacteriophage P4 Mutant Suppressin sir Mutations of Bacteriophage P2: Kim, Kyoung Jin , Sunshine, Melvin G. ¹, Six, Erich W. ¹; Journal of Microbiology 1998;36(4):262-265

Department of Microbiology, Sunmoon University; ¹Department of Microbiology, University of IowaDepartment of Microbiology, Sunmoon University; ¹Department of Microbiology, University of Iowa

Corresponding author: Kim, Kyoung Jin , Tel: 0418-530-2273,

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Abstract

Bacteriophage P4 ost1 was isolated as a suppressor mutant of P2 sir3 and identified by restriction enzyme site analysis. The mutant DNA turned out to be an imperfect P4 trimer containing deletions. It was suggested that the deletion resulted from int-mediated site-specific recombination. CsCl equilibrium density gradient experiment confirmed the genome size of P4 ostl.

Keywords: Bacteriophage P2-P4; E. coli; packaging; site-specific recombination; integrase.

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Characterization and Identification of the Bacteriophage P4 Mutant Suppressin sir Mutations of Bacteriophage P2

J. Microbiol. 1998;36(4):262-265.

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