Skip Navigation
Skip to contents

Journal of Microbiology : Journal of Microbiology

OPEN ACCESS
SEARCH
Search
Advanced Search
mobile menu button

Articles

Page Path
HOME > J. Microbiol > Volume 53(8); 2015 > Article
Journal Article
Description of a novel pectin-degrading bacterial species Prevotella pectinovora sp. nov., based on its phenotypic and genomic traits
Brigita Nograsek , Tomaz Accetto , Lijana Fanedl , Gorazd Avgustin
Journal of Microbiology 2015;53(8):503-510.
DOI: https://doi.org/10.1007/s12275-015-5142-0
Published online: July 31, 2015
University of Ljubljana, Biotechnical Faculty, Animal Science Department, Division of Microbiology and Microbial Biotechnology, Groblje 3, 1230 Domzale, SloveniaUniversity of Ljubljana, Biotechnical Faculty, Animal Science Department, Division of Microbiology and Microbial Biotechnology, Groblje 3, 1230 Domzale, Slovenia
Corresponding author:  Gorazd Avgustin , Tel: +386-1-320-3827, 
Received: 16 March 2015   • Revised: 17 June 2015   • Accepted: 19 June 2015
prev next
  • 65 Views
  • 0 Download
  • 14 Crossref
  • 22 Scopus
Full Article

Abstract

Five strictly anaerobic Gram-negative bacterial strains, P4-65, P4-76T, P5-60, P5-119, and P5-125, presumably belonging to the genus Prevotella were isolated from pig fecal samples. Strains were tested for various phenotypic traits and nearcomplete genome sequences were obtained and analyzed. Phylogenetic analysis based on 16S rRNA gene sequences and multilocus sequence analysis based on five conserved genes confirmed that the strains belong to the genus Prevotella, revealing that they represent a novel and discrete lineage distinct from other known species of this genus. The size of the genome of the isolated strains is 3?.3 Mbp, and the DNA G+C content is 47.5?8.1 mol%. The isolates are strictly anaerobic, rod-shaped with rounded ends, non-motile and non-spore-forming. The main fermentation products are succinate and acetate, with minor concentrations of isovalerate, propionate and isobutyrate. Hydrogen is also produced. Major cellular fatty acids consist of anteiso-C15:0 and iso-C15:0, and a number of additional acids are present in lower concentrations. A substantial portion of genes involved in carbohydrate utilization is devoted to pectin degradation and utilization, while those supporting growth on xylan in ruminal Prevotella could not have been revealed. On the basis of the presented results, a novel species, Prevotella pectinovora sp. nov. is proposed. The type strain is P4-76T (=DSM 29996T =ZIM B1020T).

  • Cite this Article
    Cite this Article
    export Copy Download
    Close
    Download Citation
    Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.
    Format:
    • RIS — For EndNote, ProCite, RefWorks, and most other reference management software
    • BibTeX — For JabRef, BibDesk, and other BibTeX-specific software
    Include:
    • Citation for the content below
    Description of a novel pectin-degrading bacterial species Prevotella pectinovora sp. nov., based on its phenotypic and genomic traits
    J. Microbiol. 2015;53(8):503-510.   Published online July 31, 2015
    DOI: https://doi.org/10.1007/s12275-015-5142-0
    Close
Related articles
Journal of Microbiology : Journal of Microbiology
© The Microbiological Society of Korea.
TOP