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HOME > J. Microbiol > Volume 54(7); 2016 > Article
Journal Article
Hepatitis C virus infection stimulates transforming growth factor-β1 expression through up-regulating miR-192
Ji Hyun Kim , Chang Ho Lee , Seong-Wook Lee
Journal of Microbiology 2016;54(7):520-526.
DOI: https://doi.org/10.1007/s12275-016-6240-3
Published online: June 28, 2016
Department of Molecular Biology, Research Institute of Advanced Omics, Dankook University, Yongin 16890, Republic of KoreaDepartment of Molecular Biology, Research Institute of Advanced Omics, Dankook University, Yongin 16890, Republic of Korea
Corresponding author:  Seong-Wook Lee , Tel: -, 
Received: 25 May 2016   • Revised: 7 June 2016   • Accepted: 7 June 2016
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Abstract

The objective of this study was to determine the molecular mechanisms underlying chronic liver injury and fibrosis caused by hepatitis C virus (HCV). This study revealed that miR-192 expression was induced by HCV infection without affecting viral replication. However, viral-induced miR-192 up-regulated transforming growth factor-β1 (TGF-β1) expression in liver cells at transcriptional level. TGF-β1 stimulation by HCV-induced miR-192 was caused through ZEB1 down-regulation and TGF-β1 increased miR-192 level via positive feedback pathway. Increase in miR-192 expression by HCV infection was due to HCV core protein released and/or expressed by viral infection. TGF-β1 promoter activity was also increased by HCV core protein in liver cells. Taken together, HCV infection resulted in increased TGF-β1 transcription in hepatocytes through ZEB1 down-regulation by HCV core-mediated miR-192 stimulation. Importantly, miR-192 inhibition with anti-miR-192 rescued ZEB1 expression down-regulated by HCV infection, thus reducing the level of TGF-β1 expression increased by HCV infection in hepatocytes. These results suggest a novel mechanism of HCV-mediated liver fibrogenesis with miR-192 being a potential molecular target to ameliorate viral pathogenesis.

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    Hepatitis C virus infection stimulates transforming growth factor-β1 expression through up-regulating miR-192
    J. Microbiol. 2016;54(7):520-526.   Published online June 28, 2016
    DOI: https://doi.org/10.1007/s12275-016-6240-3
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