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Electrophoretic Karyotypes of Fusarium oxysporum formae speciales
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HOME > J. Microbiol > Volume 36(1); 1998 > Article
Electrophoretic Karyotypes of Fusarium oxysporum formae speciales
Min, Byung Re 1, Kim, Kyung Ah , Choi, Yong Keel 2
Journal of Microbiology 1998;36(1):14-19

¹Department of Biiology, Sangmyung University; ²Department of Biology, Hanyang University, and Research Center for Molecular Microbiology, Seoul National University¹Department of Biiology, Sangmyung University; ²Department of Biology, Hanyang University, and Research Center for Molecular Microbiology, Seoul National University
Corresponding author:  Min, Byung Re ,
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Abstract

Chromosomal DNAs from nine strains of Fusarium oxysporum formae speciales were analysed by pulsed field gel electrophoresis (PFGE). Intact chromosomal DNA were prepared from fungal protoplasts. About 7-13 chromosomal DNA bands were separated in nine Fusarium oxysporum formaespeciales, ranging in size from 0.8 to 6.7 Mb. Considering that some of the chromosomal bands represented unresolved doublets, the total genome size was estimated to vary between at least 22.3 and 45.5 Mb. Gels run under different conditions were Southern blotted and probed with randomly selected genomic DNA from Fusarium oxysporum f. sp. lilii. Electrophoretix karyotyping revealed some differences within and between Fusarium oxysporum formae speciales.

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    Electrophoretic Karyotypes of Fusarium oxysporum formae speciales
    J. Microbiol. 1998;36(1):14-19.
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