Application of high-resolution melting analysis for differentiation of spoilage yeasts

Mine Erdem; Zülal Kesmen; Esra Özbekar; Bülent Çetin; Hasan Yetim

doi:10.1007/s12275-016-6017-8

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Application of high-resolution melting analysis for differentiation of spoilage yeasts: Mine Erdem ¹, Zülal Kesmen ¹, Esra Özbekar ¹, Bülent Çetin ², Hasan Yetim ¹; Journal of Microbiology 2016;54(9):618-625.
DOI: https://doi.org/10.1007/s12275-016-6017-8
Published online: August 31, 2016

¹Erciyes University, Faculty of Engineering, Food Engineering Department, Kayseri 38039, Turkey, ²Ataturk University, Faculty of Agriculture, Food Engineering Department, Erzurum 25240, Turkey¹Erciyes University, Faculty of Engineering, Food Engineering Department, Kayseri 38039, Turkey, ²Ataturk University, Faculty of Agriculture, Food Engineering Department, Erzurum 25240, Turkey

Corresponding author: Zülal Kesmen , Tel: -,

Received: 18 January 2016 • Revised: 24 June 2016 • Accepted: 20 July 2016

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Abstract

A new method based on high resolution melting (HRM) analysis was developed for the differentiation and classification of the yeast species that cause food spoilage. A total 134 strains belonging to 21 different yeast species were examined to evaluate the discriminative power of HRM analysis. Two different highly variable DNA regions on the 26 rRNA gene were targeted to produce the HRM profiles of each strain. HRMbased grouping was compared and confirmed by (GTG)5 rep- PCR fingerprinting analysis. All of the yeast species belonging to the genera Pichia, Candida, Kazachstania, Kluyveromyces, Debaryomyces, Dekkera, Saccharomyces, Torulaspora, Ustilago, and Yarrowia, which were produced as species-specific HRM profiles, allowed discrimination at species and/or strain level. The HRM analysis of both target regions provided successful discrimination that correlated with rep-PCR fingerprinting analysis. Consequently, the HRM analysis has the potential for use in the rapid and accurate classification and typing of yeast species isolated from different foods to determine their sources and routes as well as to prevent contamination.

Keywords: High-resolution melting analysis;yeast species;melting curve;26S rRNA gene

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Application of high-resolution melting analysis for differentiation of spoilage yeasts

J. Microbiol. 2016;54(9):618-625. Published online August 31, 2016

DOI: https://doi.org/10.1007/s12275-016-6017-8

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