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Optimization of Enterobacter cloacae (KU923381) for diesel oil degradation using Response Surface Methodology (RSM)
Sugumar Ramasamy , Arumugam Arumugam , Preethy Chandran
Journal of Microbiology 2017;55(2):104-111.
DOI: https://doi.org/10.1007/s12275-017-6265-2
Published online: January 26, 2017
Centre for Nanotechnology & Advanced Biomaterials, School of Chemical and Biotechnology, SASTRA University, IndiaCentre for Nanotechnology & Advanced Biomaterials, School of Chemical and Biotechnology, SASTRA University, India
Corresponding author:  Preethy Chandran , Tel: +91-4362-264101, 
Received: 3 June 2016   • Revised: 4 October 2016   • Accepted: 20 October 2016
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Abstract

Efficiency of Enterobacter cloacae KU923381 isolated from petroleum hydrocarbon contaminated soil was evaluated in batch culture and bioreactor mode. The isolate were screened for biofilm formation using qualitative and quantitative assays. Response surface methodology (RSM) was used to study the effect of pH, temperature, glucose concentration, and sodium chloride on diesel degradation. The predicted values for diesel oil degradation efficiency by the statistical designs are in a close agreement with experimental data (R2 = 99.66%). Degradation efficiency is increased by 36.78% at pH = 7, temperature = 35°C, glucose = 5%, and sodium chloride concentration = 5%. Under the optimized conditions, the experiments were performed for diesel oil degradation by gas chromatographic mass spectrometric analysis (GC-MS). GC-MS analysis confirmed that E. cloacae had highly degrade hexadecane, heptadecane, tridecane, and docosane by 99.71%, 99.23%, 99.66%, and 98.34% respectively. This study shows that rapid bioremoval of hydrocarbons in diesel oil is acheived by E. cloacae with abet of biofilm formation. The potential use of the biofilms for preparing trickling filters (gravel particles) for the degradation of hydrocarbons from petroleum wastes before their disposal in the open environment is highly suggested. This is the first successful attempt for artificially establishing petroleum hydrocarbon degrading bacterial biofilm on solid substrates in bioreactor.

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    Optimization of Enterobacter cloacae (KU923381) for diesel oil degradation using Response Surface Methodology (RSM)
    J. Microbiol. 2017;55(2):104-111.   Published online January 26, 2017
    DOI: https://doi.org/10.1007/s12275-017-6265-2
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