Abstract
Efficiency of Enterobacter cloacae KU923381 isolated from
petroleum hydrocarbon contaminated soil was evaluated in
batch culture and bioreactor mode. The isolate were screened
for biofilm formation using qualitative and quantitative assays.
Response surface methodology (RSM) was used to study the
effect of pH, temperature, glucose concentration, and sodium
chloride on diesel degradation. The predicted values for
diesel oil degradation efficiency by the statistical designs are
in a close agreement with experimental data (R2 = 99.66%).
Degradation efficiency is increased by 36.78% at pH = 7,
temperature = 35°C, glucose = 5%, and sodium chloride concentration
= 5%. Under the optimized conditions, the experiments
were performed for diesel oil degradation by gas chromatographic
mass spectrometric analysis (GC-MS). GC-MS
analysis confirmed that E. cloacae had highly degrade hexadecane,
heptadecane, tridecane, and docosane by 99.71%,
99.23%, 99.66%, and 98.34% respectively. This study shows
that rapid bioremoval of hydrocarbons in diesel oil is acheived
by E. cloacae with abet of biofilm formation. The potential
use of the biofilms for preparing trickling filters (gravel particles)
for the degradation of hydrocarbons from petroleum
wastes before their disposal in the open environment is highly
suggested. This is the first successful attempt for artificially
establishing petroleum hydrocarbon degrading bacterial biofilm
on solid substrates in bioreactor.
Citations
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