Evidence of the genetic diversity and clonal population structure of Oenococcus oeni strains isolated from different wine-making regions of China

Dongliang Yu; Kan Shi; Xiangyuan Wen; Fangshu Xie; Tao Wang; Shuwen Liu; Ling He

doi:10.1007/s12275-018-7568-7

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Evidence of the genetic diversity and clonal population structure of Oenococcus oeni strains isolated from different wine-making regions of China: Dongliang Yu ^1,2, Kan Shi ¹, Xiangyuan Wen ¹, Fangshu Xie ¹, Tao Wang ³, Shuwen Liu ^1,4,5, Ling He ⁶; Journal of Microbiology 2018;56(8):556-564
DOI: https://doi.org/10.1007/s12275-018-7568-7
Published online: July 25, 2018

¹College of Enology, Northwest Agriculture and Forestry University, Shaanxi 712100, P. R. China, ²Chateau Kings International Co., Ltd. Qinhuangdao, Hebei 066600, P. R. China, ³Sichuan University of Science and Engineering, Sichuan 643000, P. R. China, ⁴Shaanxi Engineering Research Center for Viti-Viniculture, Shaanxi 712100, P. R. China, ⁵Heyang Experimental and Demonstrational Stations for Grape, Shaanxi 715300, P. R. China, ⁶College of Horticulture, Northwest Agriculture and Forestry University, Shaanxi 712100, P. R. China¹College of Enology, Northwest Agriculture and Forestry University, Shaanxi 712100, P. R. China, ²Chateau Kings International Co., Ltd. Qinhuangdao, Hebei 066600, P. R. China, ³Sichuan University of Science and Engineering, Sichuan 643000, P. R. China, ⁴Shaanxi Engineering Research Center for Viti-Viniculture, Shaanxi 712100, P. R. China, ⁵Heyang Experimental and Demonstrational Stations for Grape, Shaanxi 715300, P. R. China, ⁶College of Horticulture, Northwest Agriculture and Forestry University, Shaanxi 712100, P. R. China

Corresponding author: Ling He , Tel: +86-29-8708-2613;,

Received: 28 December 2017 • Revised: 23 May 2018 • Accepted: 28 May 2018

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Abstract

Studies of the genetic diversity and population structure of Oenococcus oeni (O. oeni) strains from China are lacking compared to other countries and regions. In this study, amplified fragment length polymorphism (AFLP) and multilocus sequence typing (MLST) methods were used to investigate the genetic diversity and regional evolutionary patterns of 38 O. oeni strains isolated from different wine-making regions in China. The results indicated that AFLP was markedly more efficient than MLST for typing O. oeni strains. AFLP distinguished 37 DNA patterns compared to 7 sequence types identified using MLST, corresponding to discriminatory indices of 0.999 and 0.602, respectively. The AFLP results revealed a high level of genetic diversity among the O. oeni strains from different regions of China, since two subpopulations and an intraspecific homology higher than 60% were observed. Phylogenetic analysis of the O. oeni strains using the MLST method also identified two major phylogroups, which were differentiated into two distinct clonal complexes by minimum spanning tree analysis. Neither intragenic nor intergenic recombination verified the existence of the clonal population structure of the O. oeni strains.

Keywords: Oenococcus oeni; amplified fragment length polymorphism; multilocus sequence typing; genetic diversity; clonal population structure

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Evidence of the genetic diversity and clonal population structure of Oenococcus oeni strains isolated from different wine-making regions of China

J. Microbiol. 2018;56(8):556-564. Published online July 25, 2018

DOI: https://doi.org/10.1007/s12275-018-7568-7

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