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HOME > J. Microbiol > Volume 56(11); 2018 > Article
Article
Photodynamic antimicrobial activity of new porphyrin derivatives against methicillin resistant Staphylococcus aureus
Hüseyin Ta&# 351;lı1, Ay&# 351;e Akbıyık2, Nermin Topalo&# 287;lu3, Vildan Alptüzün 4
Journal of Microbiology 2018;56(11):828-837.
DOI: https://doi.org/10.1007/s12275-018-8244-7
Published online: October 24, 2018
1Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Ege University, 35100 Izmir, Turkey, 2Department of Nursing, Faculty of Health Sciences, Izmir Katip Celebi University, 35620 Izmir, Turkey, 3Department of Biomedical Engineering, Faculty of Engineering and Architecture Izmir Katip Celebi University, 35620 Izmir, Turkey, 4Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Ege University, 35100 Izmir, Turkey
Received: 4 May 2018   • Revised: 2 August 2018   • Accepted: 2 August 2018
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Abstract

Methicillin resistant Staphylococcus aureus (MRSA) with multiple drug resistance patterns is frequently isolated from skin and soft tissue infections that are involved in chronic wounds. Today, difficulties in the treatment of MRSA associated infections have led to the development of alternative approaches such as antimicrobial photodynamic therapy. This study aimed to investigate photoinactivation with cationic porphyrin derivative compounds against MRSA in in-vitro conditions. In the study, MRSA clinical isolates with different antibiotic resistance profiles were used. The newly synthesized cationic porphyrin derivatives (PM, PE, PPN, and PPL) were used as photosensitizer, and 655 nm diode laser was used as light source. Photoinactivation experiments were performed by optimizing energy doses and photosensitizer concentrations. In photoinactivation experiments with different energy densities and photosensitizer concentrations, more than 99% reduction was achieved in bacterial cell viability. No decrease in bacterial survival was observed in control groups. It was determined that there was an increase in photoinactivation efficiency by increasing the energy dose. At the energy dose of 150 J/cm2 a survival reduction of over 6.33 log10 was observed in each photosensitizer type. While 200 μM PM concentration was required for this photoinactivation, 12.50 μM was sufficient for PE, PPN, and PPL. In our study, antimicrobial photodynamic therapy performed with cationic porphyrin derivatives was found to have potent antimicrobial efficacy against multidrug resistant S. aureus which is frequently isolated from wound infections.

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    Photodynamic antimicrobial activity of new porphyrin derivatives against methicillin resistant Staphylococcus aureus
    J. Microbiol. 2018;56(11):828-837.   Published online October 24, 2018
    DOI: https://doi.org/10.1007/s12275-018-8244-7
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