Skip Navigation
Skip to contents

Journal of Microbiology : Journal of Microbiology

OPEN ACCESS
SEARCH
Search
Advanced Search
mobile menu button

Articles

Page Path
HOME > J. Microbiol > Volume 56(11); 2018 > Article
Journal Article
Comparative genomic analysis of pyrene-degrading Mycobacterium species: Genomic islands and ring-hydroxylating dioxygenases involved in pyrene degradation
Dae-Wi Kim , Kihyun Lee , Do-Hoon Lee , Chang-Jun Cha
Journal of Microbiology 2018;56(11):798-804.
DOI: https://doi.org/10.1007/s12275-018-8372-0
Published online: October 24, 2018
Department of Systems Biotechnology and Center for Antibiotic Resistome, Chung-Ang University, Anseong 17456, Republic of KoreaDepartment of Systems Biotechnology and Center for Antibiotic Resistome, Chung-Ang University, Anseong 17456, Republic of Korea
Corresponding author:  Chang-Jun Cha , Tel: +82-31-670-4840, 
Received: 9 July 2018   • Revised: 9 August 2018   • Accepted: 13 August 2018
prev next
  • 42 Views
  • 0 Download
  • 21 Crossref
  • 24 Scopus
Full Article

Abstract

The genome sequences of two pyrene-degrading bacterial strains of Mycobacterium spp. PYR10 and PYR15, isolated from the estuarine wetland of the Han river, South Korea, were determined using the PacBio RS II sequencing platform. The complete genome of strain PYR15 was 6,037,017 bp in length with a GC content of 66.5%, and contained 5,933 protein- coding genes. The genome of strain PYR10 was 5,999,427 bp in length with a GC content of 67.7%, and contained 5,767 protein-coding genes. Based on the average nucleotide identity values, these strains were designated as M. gilvum PYR10 and M. pallens PYR15. A genomic comparison of these pyrene-degrading Mycobacterium strains with pyrene- non-degrading strains revealed that the genomes of pyrene-degrading strains possessed similar repertoires of ringhydroxylating dioxygenases (RHDs), including the pyrenehydroxylating dioxygenases encoded by nidA and nidA3, which could be readily distinguished from those of pyrenenon- degraders. Furthermore, genomic islands, containing catabolic gene clusters, were shared only among the pyrenedegrading Mycobacterium strains and these gene clusters contained RHD genes, including nidAB and nidA3B3. Our genome data should facilitate further studies on the evolution of the polycyclic aromatic hydrocarbon-degradation pathways in the genus Mycobacterium.

  • Cite this Article
    Cite this Article
    export Copy Download
    Close
    Download Citation
    Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.
    Format:
    • RIS — For EndNote, ProCite, RefWorks, and most other reference management software
    • BibTeX — For JabRef, BibDesk, and other BibTeX-specific software
    Include:
    • Citation for the content below
    Comparative genomic analysis of pyrene-degrading Mycobacterium species: Genomic islands and ring-hydroxylating dioxygenases involved in pyrene degradation
    J. Microbiol. 2018;56(11):798-804.   Published online October 24, 2018
    DOI: https://doi.org/10.1007/s12275-018-8372-0
    Close
Related articles
Journal of Microbiology : Journal of Microbiology
© The Microbiological Society of Korea.
TOP