Abstract
Hypocrellin A (HA) is a perylenequinone (PQ) isolated from
Shiraia bambusicola that shows antiviral and antitumor activities,
but its application is limited by the low production
from wild fruiting body. A gene overexpressing method was
expected to augment the production rate of HA in S. bambusicola.
However, the application of this molecular biology
technology in S. bambusicola was impeded by a low genetic
transformation efficiency and little genomic information. To
enhance the plasmid transformant ratio, the Polyethylene
Glycol-mediated transformation system was established and
optimized. The following green fluorescent protein (GFP)
analysis showed that the gene fusion expression system we
constructed with a GAPDH promoter Pgpd1 and a rapid 2A
peptide was successfully expressed in the S. bambusicola S4201
strain. We successfully obtained the HA high-producing strains
by overexpressing O-methyltransferase/FAD-dependent monooxygenase
gene (mono) and the hydroxylase gene (hyd),
which were the essential genes involved in our putative HA
biosynthetic pathway. The overexpression of these two genes
increased the production of HA by about 200% and 100%,
respectively. In general, this study will provide a basis to identify
the genes involved in the hypocrellin A biosynthesis. This
improved transformation method can also be used in genetic
transformation studies of other fungi.
Citations
Citations to this article as recorded by
-
Optimisation of hypocrellin production in
Shiraia
-like fungi via genetic modification involving a transcription factor gene and a putative monooxygenase gene
Zi-Min Lu, Run-Tong Zhang, Xiao-Bo Huang, Xue-Ting Cao, Xiao-Ye Shen, Li Fan, Cheng-Lin Hou
Mycology.2024; 15(2): 272. CrossRef - Production of fungal hypocrellin photosensitizers: Exploiting bambusicolous fungi and elicitation strategies in mycelium cultures
Xin Ping Li, Wen Hao Shen, Jian Wen Wang, Li Ping Zheng
Mycology.2024; : 1. CrossRef - Urea-Induced Enhancement of Hypocrellin A Synthesis in Shiraia bambusicola GDMCC 60438: Strategies and Mechanisms
Yanbo Tang, Yongdi Wen, Xiang Zhang, Qian Gao, Fuqiang Yu, Zhenqiang Wu, Xiaofei Tian
Fermentation.2024; 10(8): 381. CrossRef - Advancements and Future Prospects in Hypocrellins Production and Modification for Photodynamic Therapy
Xiang Zhang, Qiulin Wei, Liwen Tian, Zhixian Huang, Yanbo Tang, Yongdi Wen, Fuqiang Yu, Xiaoxiao Yan, Yunchun Zhao, Zhenqiang Wu, Xiaofei Tian
Fermentation.2024; 10(11): 559. CrossRef - Biosynthesis of Natural and Unnatural Perylenequinones for Drug Development
Zengping Su, Yan Zhang, Zhenbo Yuan, Yijian Rao
ChemMedChem.2024;[Epub] CrossRef - Heat stress enhanced perylenequinones biosynthesis of Shiraia sp. Slf14(w) through nitric oxide formation
Chenglong Xu, Wenxi Lin, Yunni Chen, Boliang Gao, Zhibin Zhang, Du Zhu
Applied Microbiology and Biotechnology.2023; 107(11): 3745. CrossRef - Biotechnological production and potential applications of hypocrellins
Zhuanying Bao, Yunchang Xie, Chenglong Xu, Zhibin Zhang, Du Zhu
Applied Microbiology and Biotechnology.2023; 107(21): 6421. CrossRef - L-Arginine enhanced perylenequinone production in the endophytic fungus Shiraia sp. Slf14(w) via NO signaling pathway
Yunni Chen, Chenglong Xu, Huilin Yang, Zhenying Liu, Zhibin Zhang, Riming Yan, Du Zhu
Applied Microbiology and Biotechnology.2022; 106(7): 2619. CrossRef - Advances and perspectives on perylenequinone biosynthesis
Huaxiang Deng, Xinxin Liang, Jinbin Liu, Xiaohui Zheng, Tai-Ping Fan, Yujie Cai
Frontiers in Microbiology.2022;[Epub] CrossRef - Temperature-responsive regulation of the fermentation of hypocrellin A by Shiraia bambusicola (GDMCC 60438)
Yongdi Wen, Baosheng Liao, Xiaoxiao Yan, Zhenqiang Wu, Xiaofei Tian
Microbial Cell Factories.2022;[Epub] CrossRef - Microbial production of nematicidal agents for controlling plant-parasitic nematodes
Jaemin Seong, Jongoh Shin, Kangsan Kim, Byung-Kwan Cho
Process Biochemistry.2021; 108: 69. CrossRef - Nitric oxide donor sodium nitroprusside-induced transcriptional changes and hypocrellin biosynthesis of Shiraia sp. S9
Yan Jun Ma, Xin Ping Li, Yue Wang, Jian Wen Wang
Microbial Cell Factories.2021;[Epub] CrossRef - Nitric oxide regulates perylenequinones biosynthesis in Shiraia bambusicola S4201 induced by hydrogen peroxide
Ning Zhao, Yingying Yu, Yunxia Yue, Mingzhu Dou, Bingjing Guo, Shuzhen Yan, Shuanglin Chen
Scientific Reports.2021;[Epub] CrossRef - Current State and Future Directions of Genetics and Genomics of Endophytic Fungi for Bioprospecting Efforts
Rosa Sagita, Wim J. Quax, Kristina Haslinger
Frontiers in Bioengineering and Biotechnology.2021;[Epub] CrossRef - Global identification of alternative splicing in Shiraia bambusicola and analysis of its regulation in hypocrellin biosynthesis
Xin-Yao Liu, Li Fan, Jian Gao, Xiao-Ye Shen, Cheng-Lin Hou
Applied Microbiology and Biotechnology.2020; 104(1): 211. CrossRef - Improved A40926 production from Nonomuraea gerenzanensis using the promoter engineering and the co-expression of crucial genes
Huijun Dong, Xue Yue, Bingyu Yan, Wen Gao, Shuai Wang, Yongquan Li
Journal of Biotechnology.2020; 324: 28. CrossRef - Adding bamboo charcoal powder to Shiraia bambusicola preculture improves hypocrellin A production
Xin Ping Li, Yan Jun Ma, Jian Wen Wang
Sustainable Chemistry and Pharmacy.2019; 14: 100191. CrossRef - Efficient agrobacterium-mediated transformation ofShiraia bambusicolaand activation of a specific transcription factor for hypocrellin production
Tong Li, Cheng-Lin Hou, Xiao-Ye Shen
Biotechnology & Biotechnological Equipment.2019; 33(1): 1365. CrossRef - Response mechanism of hypocrellin colorants biosynthesis by Shiraia bambusicola to elicitor PB90
Wen Du, Chunlong Sun, Baogui Wang, Yanmei Wang, Bin Dong, Junhua Liu, Jiangbao Xia, Wenjun Xie, Jun Wang, Jingkuan Sun, Xuehong Liu, Hongguo Wang
AMB Express.2019;[Epub] CrossRef
MSK
Cite this Article
