Abstract
Chitin is the most abundant biopolymer in marine environments.
To facilitate its utilization, our laboratory screened
marine-derived fungal strains for chitinolytic activity. One
chitinolytic strain isolated from seawater, designated YS2-2,
was identified as Acremonium species based on morphological
and phylogenetic analyses. Acremonium species are cosmopolitan
fungi commonly isolated from both terrestrial and
marine environments, but their chitinolytic activity is largely
unknown. The extracellular crude enzyme of YS2-2 exhibited
optimum chitinolytic activity at pH 6.0–7.6, 23–45°C, and
1.5% (w/v) NaCl. Degenerate PCR revealed the partial cDNA
sequence of a putative chitinase gene, chiA, in YS2-2. The
expression of chiA was dramatically induced in response to
1% (w/v) colloidal chitin compared to levels under starvation,
chitin powder, and glucose conditions. Moreover, the chiA
transcript levels were positively correlated with chitinolytic
activities under various colloidal chitin concentrations, suggesting
that ChiA mediates chitinolytic activity in this strain.
Our results provide a basis for additional studies of marinederived
chitinolytic fungi aimed at improving industrial applications.
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