Abstract
KatA is the major catalase required for hydrogen peroxide
(H2O2) resistance and acute virulence in Pseudomonas aeruginosa
PA14, whose transcription is governed by its dual
promoters (katAp1 and katAp2). Here, we observed that KatA
was not required for acute virulence in another wild type P.
aeruginosa strain, PAO1, but that PAO1 exhibited higher
KatA expression than PA14 did. This was in a good agreement
with the observation that PAO1 was more resistant
than PA14 to H2O2 as well as to the antibiotic peptide, polymyxin
B (PMB), supposed to involve reactive oxygen species
(ROS) for its antibacterial activity. The higher KatA expression
in PAO1 than in PA14 was attributed to both katAp1
and katAp2 transcripts, as assessed by S1 nuclease mapping.
In addition, it was confirmed that the PMB resistance is attributed
to both katAp1 and katAp2 in a complementary manner
in PA14 and PAO1, by exploiting the promoter mutants
for each -10 box (p1m, p2m, and p1p2m). These results provide
an evidence that the two widely used P. aeruginosa strains
display different virulence mechanisms associated with OxyR
and Anr, which need to be further characterized for better
understanding of the critical virulence pathways that may
differ in various P. aeruginosa strains.
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