Severe fever with thrombocytopenia syndrome (SFTS) is being
reported annually in South Korea since its first detection
there in 2010. The causal agent is a negative-strand RNA
virus 80–100 nm in diameter. It causes fever, thrombocytopenia,
leukocytopenia, gastrointestinal symptoms, and neural
symptoms. The mortality rate of SFTS was 32.6% among 172
case
s reported from 2012 to 2015 in South Korea. Thus, is
necessary to develop an effective diagnostic method that selectively
identifies the isolates circulating in South Korea. The
real-time reverse transcription loop-mediated isothermal amplification
(RT-LAMP) assay is a simple, rapid, and sensitive
approach for molecular diagnosis. Here, we designed novel
primers for this assay and found that the technique had very
high specificity, sensitivity, and efficiency. This real-time RTLAMP
approach using the novel primers developed herein
can be applied for early diagnosis of SFTSV strains in South
Korea to reduce the mortality rate of SFTS.