Expression of Human Mitochondiral Aldehyde Dehydrogenase 2 in Mammalian Cells using Vaccinia Virus-T7 RNA Polymerase

Kang, Su Min; Yoo, Seung Ku; Lee, Ki Whan


Expression of Human Mitochondiral Aldehyde Dehydrogenase 2 in Mammalian Cells using Vaccinia Virus-T7 RNA Polymerase: Kang, Su Min , Yoo, Seung Ku , Lee, Ki Whan; Journal of Microbiology 1999;37(1):41-44

Department of Biotechnology, College of Engineering and Bioproducts Research Center, Yonsei UniversityDepartment of Biotechnology, College of Engineering and Bioproducts Research Center, Yonsei University

Corresponding author: Yoo, Seung Ku , Tel: 82-2-361-2887,

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Abstract

Human mitochondrial aldehyde dehydrogenase 2 (ALDH2) is mainly responsible for oxidation of acetaldehyde generated during alcohol oxidation in vivo. A full-length cDNA of human liver ALDH2 was successfully expressed using a vaccinia virus-T7 RNA polymerase system. The expressed ALDH2 had an enzymatic activity as high as the native human liver ALDH2 enzyme.

Keywords: Acetaldehyde; human mitochondrial aldehyde dehydrogenase 2(ALDH2); vaccinia virus T7 RNA polymerase

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Expression of Human Mitochondiral Aldehyde Dehydrogenase 2 in Mammalian Cells using Vaccinia Virus-T7 RNA Polymerase

J. Microbiol. 1999;37(1):41-44.

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