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HOME > J. Microbiol > Volume 59(5); 2021 > Article
Journal Article
Role of melatonin in murine “restraint stress”-induced dysfunction of colonic microbiota
Rutao Lin , Zixu Wang , Jing Cao , Ting Gao , Yulan Dong , Yaoxing Chen
Journal of Microbiology 2021;59(5):500-512.
DOI: https://doi.org/10.1007/s12275-021-0305-7
Published online: February 25, 2021
Neurobiology Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, P. R. ChinaNeurobiology Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, P. R. China
Corresponding author:  Yulan Dong , Tel: +86-10-62733778;, 
Yaoxing Chen , Tel: +86-10-62733778;, 
Received: 15 June 2020   • Revised: 16 December 2020   • Accepted: 5 January 2021
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Abstract

Intestinal diseases caused by physiological stress have become a severe public health threat worldwide. Disturbances in the gut microbiota-host relationship have been associated with irritable bowel disease (IBD), while melatonin (MT) has antiinflammatory and antioxidant effects. The objective of this study was to investigate the mechanisms by which MT-mediated protection mitigated stress-induced intestinal microbiota dysbiosis and inflammation. We successfully established a murine restraint stress model with and without MT supplementation. Mice subjected to restraint stress had significantly elevated corticosterone (CORT) levels, decreased MT levels in their plasma, elevated colonic ROS levels and increased bacterial abundance, including Bacteroides and Tyzzerella, in their colon tract, which led to elevated expression of Toll-like receptor (TLR) 2/4, p-P65 and p-IκB. In contrast, supplementation with 20 mg/kg MT reversed the elevation of the plasma CORT levels, downregulated the colon ROS levels and inhibited the changes in the intestinal microbiota induced by restraint stress. These effects, in turn, inhibited the activities of TLR2 and TLR4, p-P65 and p-IκB, and decreased the inflammatory reaction induced by restraint stress. Our results suggested that MT may mitigate “restraint stress”-induced colonic microbiota dysbiosis and intestinal inflammation by inhibiting the activation of the NF-κB pathway.

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    Role of melatonin in murine “restraint stress”-induced dysfunction of colonic microbiota
    J. Microbiol. 2021;59(5):500-512.   Published online February 25, 2021
    DOI: https://doi.org/10.1007/s12275-021-0305-7
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