Improved tolerance of recombinant Chlamydomonas rainhardtii with putative 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase from Pyropia yezoensis to nitrogen starvation

Seo-jeong Park; Joon Woo Ahn; Jong-il Choi

doi:10.1007/s12275-022-1491-7

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Improved tolerance of recombinant Chlamydomonas rainhardtii with putative 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase from Pyropia yezoensis to nitrogen starvation: Seo-jeong Park ¹, Joon Woo Ahn ², Jong-il Choi ¹; Journal of Microbiology 2022;60(1):63-69
DOI: https://doi.org/10.1007/s12275-022-1491-7
Published online: December 29, 2021

¹Department of Biotechnology and Bioengineering, Chonnam National University, Gwangju 61186, Republic of Korea, ²Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of Korea¹Department of Biotechnology and Bioengineering, Chonnam National University, Gwangju 61186, Republic of Korea, ²Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of Korea

Corresponding author: Jong-il Choi , Tel: +82-62-530-1846,

Received: 23 September 2021 • Revised: 16 November 2021 • Accepted: 17 November 2021

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Abstract

In a previous study, a putative 2-amino-3-carboxymuconate- 6-semialdehyde decarboxylase (ACMSD) was highly expressed in a mutant strain of Pyropia yezoensis, which exhibited an improved growth rate compared to its wild strain. To investigate the functional role of the putative ACMSD (Pyacmsd) of P. yezoensis, the putative Pyacmsd was cloned and expressed in Chlamydomonas reinhardtii. Recombinant C. reinhardtii cells with Pyacmsd (Cr_Pyacmsd) exhibited enhanced tolerance compared to control C. reinhardtii cells (Cr_control) under nitrogen starvation. Notably, Cr_Pyacmsd cells showed accumulation of lipids in nitrogen-enriched conditions. These
results
demonstrate the role of Pyacmsd in the generation of acetyl-coenzyme A. Thus, it can be used to enhance the production of biofuel using microalgae such as C. reinhardtii and increase the tolerance of other biological systems to nitrogendeficient conditions.

Keywords: Autism spectrum disorder;Detoxification;Sleeping disorder;Washed microbiota transplantation

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Improved tolerance of recombinant Chlamydomonas rainhardtii with putative 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase from Pyropia yezoensis to nitrogen starvation

J. Microbiol. 2022;60(1):63-69. Published online December 29, 2021

DOI: https://doi.org/10.1007/s12275-022-1491-7

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