Flaviflexus equikiangi sp. nov. isolated from faeces of Equus kiang (Tibetan wild ass) and carrying a class 1 integron gene cassette in its genome

Caixin Yang; Xingxing Lian; Yanpeng Cheng; Yifan Jiao; Jing Yang; Kui Dong; Shan Lu; Xin-He Lai; Dong Jin; Han Zheng; Ji Pu; Suping Wang; Liyun Liu; Jianguo Xu

doi:10.1007/s12275-022-1673-3

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Flaviflexus equikiangi sp. nov. isolated from faeces of Equus kiang (Tibetan wild ass) and carrying a class 1 integron gene cassette in its genome: Caixin Yang ^1,2, Xingxing Lian ^1,2, Yanpeng Cheng ^1,2,3, Yifan Jiao ^1,2, Jing Yang ^2,4,5, Kui Dong ^1,2,6, Shan Lu ^2,4,5, Xin-He Lai ⁷, Dong Jin ^2,4,5, Han Zheng ^2,4, Ji Pu ², Suping Wang ¹, Liyun Liu ^2,5, Jianguo Xu ^1,2,4,5,8; Journal of Microbiology 2022;60(6):585-593
DOI: https://doi.org/10.1007/s12275-022-1673-3
Published online: April 18, 2022

¹Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan, Shanxi 030001, P. R. China, ²State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, P. R. China, ³Shenzhen Center for Disease Control and Prevention, Shenzhen 518000, PR China, ⁴Shanghai Institute for Emerging and Re-emerging Infectious Diseases, Shanghai Public Health Clinical Center, Shanghai 201508, P. R. China, ⁵Research Units of Discovery of Unknown Bacteria and Function, Chinese Academy of Medical Sciences, Beijing 102206, P. R. China, ⁶Shanxi Eye Hospital, Taiyuan, Shanxi 030001, P. R. China, ⁷Henan Key Laboratory of Biomolecular Recognition and Sensing, College of Chemistry and Chemical Engineering, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, Shangqiu Normal University, Shangqiu 476000, P. R. China¹Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan, Shanxi 030001, P. R. China, ²State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, P. R. China, ³Shenzhen Center for Disease Control and Prevention, Shenzhen 518000, PR China, ⁴Shanghai Institute for Emerging and Re-emerging Infectious Diseases, Shanghai Public Health Clinical Center, Shanghai 201508, P. R. China, ⁵Research Units of Discovery of Unknown Bacteria and Function, Chinese Academy of Medical Sciences, Beijing 102206, P. R. China, ⁶Shanxi Eye Hospital, Taiyuan, Shanxi 030001, P. R. China, ⁷Henan Key Laboratory of Biomolecular Recognition and Sensing, College of Chemistry and Chemical Engineering, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, Shangqiu Normal University, Shangqiu 476000, P. R. China

Corresponding author: Jianguo Xu ,

Received: 27 December 2021 • Revised: 17 February 2022 • Accepted: 3 March 2022

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Abstract

Two Gram-stain-positive, catalase-negative, non-spore-forming, cocci-shaped strains (dk850T and JY899) were isolated from the feces of Equus kiang in the Qinghai-Tibet Plateau of China. 16S rRNA gene sequence-based phylogenetic analyses showed that strains dk850T and JY899 belong to the genus Flaviflexus, closest to F. salsibiostraticola KCTC 33148T, F. ciconiae KCTC 49253T and F. huanghaiensis H5T. The DNA G + C content of strain dk850T was 62.9%. The digital DNADNA hybridization values of strain dk850T with the closely related species were below the 70% threshold for species demarcation. The two strains grew best at 28°C on brain heart infusion (BHI) agar with 5% sheep blood. All strains had C18:1ω9c and C16:0 as the major cellular fatty acids. MK-9(H4) was the major menaquinone in strain dk850T. The major polar lipids included diphosphatidylglycerol and an unidentified phospholipid. Strains dk850T and JY899 were identified as carrying a class 1 integron containing the aminoglycoside resistance gene aadA11, both strains were resistant to spectinomycin and streptomycin. Based on several lines of evidence from phenotypic and phylogenetic analyses, strains dk850T and JY899 represent a novel species of the genus Flaviflexus, for which the name Flaviflexus equikiangi sp. nov. is proposed. The type strain is dk850T (= CGMCC 1.16593T = JCM 33598T).

Keywords: 16S rRNA gene sequence;Mesorhizobium denitrificans;polyphasic taxomony;sludge

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Flaviflexus equikiangi sp. nov. isolated from faeces of Equus kiang (Tibetan wild ass) and carrying a class 1 integron gene cassette in its genome

J. Microbiol. 2022;60(6):585-593. Published online April 18, 2022

DOI: https://doi.org/10.1007/s12275-022-1673-3

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