Whole-genome sequencing analysis of Shiga toxin-producing Escherichia coli O22:H8 isolated from cattle prediction pathogenesis and colonization factors and position in STEC universe phylogeny

Wanderson Marques Da Silva; Mariano Larzabal; Flavia Figueira Aburjaile; Nahuel Riviere; Luisina Martorelli; James Bono; Ariel Amadio; Angel Cataldi

doi:10.1007/s12275-022-1616-z

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Whole-genome sequencing analysis of Shiga toxin-producing Escherichia coli O22:H8 isolated from cattle prediction pathogenesis and colonization factors and position in STEC universe phylogeny: Wanderson Marques Da Silva ¹, Mariano Larzabal ¹, Flavia Figueira Aburjaile ², Nahuel Riviere ¹, Luisina Martorelli ³, James Bono ⁴, Ariel Amadio ⁵, Angel Cataldi ¹; Journal of Microbiology 2022;60(7):689-704
DOI: https://doi.org/10.1007/s12275-022-1616-z
Published online: June 22, 2022

¹Agrobiotechnology and Molecular Biology Institute (IABIMO-INTA/CONICET), Hurlingham B1686IGC, Argentina, ²Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte 31270-901, Brazil, ³Institute of Veterinary Pathobiology (IPEVET-INTA/CONICET), Hurlingham B1686WAA, Argentina, ⁴U.S Meat Animal Research Center, Agricultural Research Service, U.S. Department of Agriculture, Clay Center, Nebraska 68933, USA, ⁵Dairy Chain Research Institute (IDICAL-INTA/CONICET), Rafaela 2300, Santa Fe S2300, Argentina¹Agrobiotechnology and Molecular Biology Institute (IABIMO-INTA/CONICET), Hurlingham B1686IGC, Argentina, ²Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte 31270-901, Brazil, ³Institute of Veterinary Pathobiology (IPEVET-INTA/CONICET), Hurlingham B1686WAA, Argentina, ⁴U.S Meat Animal Research Center, Agricultural Research Service, U.S. Department of Agriculture, Clay Center, Nebraska 68933, USA, ⁵Dairy Chain Research Institute (IDICAL-INTA/CONICET), Rafaela 2300, Santa Fe S2300, Argentina

Corresponding author: Wanderson Marques Da Silva , Tel: +54-11-4621-1127(#3603),

Received: 29 November 2021 • Revised: 25 February 2022 • Accepted: 24 March 2022

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Abstract

Shiga toxin-producing Escherichia coli (STEC) is a foodborne pathogen capable of causing illness in humans. In a previous study, our group showed that a STEC isolate belonging to O22:H8 serotype (strain 154) can interfere with STEC O157:H7 colonization both in vitro and in vivo. Using whole-genome sequencing and genomic comparative, we predicted a subset of genes acquired by O22:H8 strain 154 through horizontal gene transfer that might be responsible for the phenotype previously described by our group. Among them were identified genes related to the pathogenesis of non-LEE (locus of enterocyte effacement) STEC, specific metabolic processes, antibiotic resistance and genes encoding for the T6SS-1 that is related to inter-bacterial competition. In addition, we showed that this strain carries stx1c and stx2dact, a mucus-inducible variant. The results obtained in this study provide insights into STEC genomic plasticity and the importance of genomic islands in the adaptation and pathogenesis of this pathogen.

Keywords: Ganoderma boninense;Ganoderma mycelium;antibacterial activity;disc diffusion assay;liquid-liquid extraction;phytochemical analysis;solvent extraction

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Whole-genome sequencing analysis of Shiga toxin-producing Escherichia coli O22:H8 isolated from cattle prediction pathogenesis and colonization factors and position in STEC universe phylogeny

J. Microbiol. 2022;60(7):689-704. Published online June 22, 2022

DOI: https://doi.org/10.1007/s12275-022-1616-z

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