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Use of the Yeast 1.5-Hybrid System to Detect DNA-Protein-Protein Interactions
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HOME > J. Microbiol > Volume 38(2); 2000 > Article
Use of the Yeast 1.5-Hybrid System to Detect DNA-Protein-Protein Interactions
Sook-Kyung Kim , Jin Hee Han
Journal of Microbiology 2000;38(2):113-116

Laboratory of Fermentation Microbiology and Biotechnology, School of Biological Sciences, Seoul National University, Seoul 151-742, KoreaLaboratory of Fermentation Microbiology and Biotechnology, School of Biological Sciences, Seoul National University, Seoul 151-742, Korea
Corresponding author:  Sook-Kyung Kim , Tel: 82-2-880-6705, 
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Abstract

Escherichia coli F plasmid partition apparatus is composed of two trans-acting proteins (SopA and SopB) and one cis-acting DNA sequence (sopC). The SopB-sopC complex has been suggested to serve a centromere-like function through its interaction with chromosomally encoded proteins which remain to be identified. In this paper, we are introducing a new yeast 1.5-hybrid system which assembles the two-hybrid and one-hybrid system as a mean to find an additional component of the F plasmid partition system, interacting with DNA (sopC)-bound SopB protein. The result indicates that this system is a promising one, capable of selecting an interacting component.

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    Use of the Yeast 1.5-Hybrid System to Detect DNA-Protein-Protein Interactions
    J. Microbiol. 2000;38(2):113-116.
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