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HOME > J. Microbiol > Volume 62(12); 2024 > Article
Journal Article
CalR Inhibits the Swimming Motility and Polar Flagellar Gene Expression in Vibrio parahaemolyticus
Jingyang Chang1,2, Yining Zhou1,2, Miaomiao Zhang1, Xue Li1, Nan Zhang1,2, Xi Luo1, Bin Ni2,3, Haisheng Wu3, Renfei Lu1, Yiquan Zhang1,3
Journal of Microbiology 2024;62(12):1125-1132
DOI: https://doi.org/10.1007/s12275-024-00179-0
Published online: December 6, 2024
1Department of Clinical Laboratory, Nantong Third People’s Hospital, Affiliated Nantong Hospital 3 of Nantong University, Nantong, 226006, Jiangsu, People’s Republic of China
2Department of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, People’s Republic of China
3Qinghai Institute for Endemic Disease Prevention and Control, Xining, 811602, Qinghai, People’s Republic of China
Corresponding author:  Haisheng Wu,
Email: qh_whs@163.com
Renfei Lu,
Email: rainman78@163.com
Yiquan Zhang,
Email: zhangyiquanq@163.com

Jingyang Chang and Yining Zhou contributed equally to this study as co-first authors.

Received: 19 July 2024   • Revised: 14 September 2024   • Accepted: 29 September 2024
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Vibrio parahaemolyticus has two flagellar systems, the polar flagellum and lateral flagella, which are both intricately regulated by a multitude of factors. CalR, a LysR-type transcriptional regulator, is sensitive to calcium (Ca) and plays a crucial role in regulating the virulence and swarming motility of V. parahaemolyticus. In this study, we have demonstrated that the deletion of calR significantly enhances the swimming motility of V. parahaemolyticus under low Ca conditions but not under high Ca conditions or in the absence of Ca. CalR binds to the regulatory DNA regions of flgM, flgA, and flgB, which are located within the polar flagellar gene loci, with the purpose of repressing their transcription. Additionally, it exerts an indirect negative control over the transcription of flgK. The overexpression of CalR in Escherichia coli resulted in a reduction in the expression levels of flgM, flgA, and flgB, while having no impact on the expression of flgK. In summary, this research demonstrates that the negative regulation of V. parahaemolyticus swimming motility by CalR under low Ca conditions is achieved through its regulation on the transcription of polar flagellar genes.

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    CalR Inhibits the Swimming Motility and Polar Flagellar Gene Expression in Vibrio parahaemolyticus
    J. Microbiol. 2024;62(12):1125-1132.   Published online December 6, 2024
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