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- P22-Based Challenge Phage Constructs to Study DNA-Protein Interactions between the [sigma]^54-Dependent Promoter, dctA, and Its Transcriptional Regulators
- Eungbin Kim 1, Daeyou Kim 2, Joon Haeng Lee 3
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Journal of Microbiology 2000;38(3):176-179
1 Department of Biology and Institute of Bioscience and Biotechnology, Yonsei University, Seoul 120-749, Korea; 2 Department of Genetic Engineering, Y1 Department of Biology and Institute of Bioscience and Biotechnology, Yonsei University, Seoul 120-749, Korea; 2 Department of Genetic Engineering, Y
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Corresponding author:
Joon Haeng Lee , Tel: 82-2-361-8461,
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Abstract
A challenge phage system was used to study the DNA-protein interaction between C_4 -dicarboylic acid transport protein D (DCTD) or [sigma]^54 , and a [sigma]^54 -dependent promoter, dctAp. R. meliloti dctA promoter regulatory region replaced the O_mnt site on the phage. S. typhimurium strains overproducing either DCTD or [sigma]^54 directed this challenge phage towards lysogeny, indicating that DCTD or E[sigma]^54 recognized the dctA promoter on the phage and repressed transcription of the ant gene. These challenge phage constructs will be useful for examining interactions between DCTD (or [sigma]^54 ) and the dctA promoter region.
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