Abstract

We have identified hemolysin rendering virulency of Vibrio anguilarum grown at 23℃ which was evaluated on human RBCs. Hemolysin itself was separated as a single band on non-denaturing gel electrophoresis. Vibrio hemolysin was destroyed by trypsin and proteinase K and was heat labile. Optimal pH for activity was around pH 6 while pI of the molecule was recognized as 5.7, with relative distance (R_f) on non-denaturing gel was 0.7. Addition of EDTA and FeCI₃drew the possibility that the production of hemolysin was mainly induced to overcome iron deficiency inside host animals upon infection.

• Keywords: Vibrio anguillarum; hemolysin; iron deficiency