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- Isolation, Identification and Characterization of Vancomycin-Resistant Enterococci from Raw Milk
- Sung-Sook Choi 1, Bong-Su Kim 2, Nam-Joo Ha
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Journal of Microbiology 2002;40(2):170-172
*Department of Pharmacy, Sahmyook University, Seoul 139-742, Korea; 1 Department of Food Science, Sahmyook College, Seoul 139-742, Korea; 2 Laboratory*Department of Pharmacy, Sahmyook University, Seoul 139-742, Korea; 1 Department of Food Science, Sahmyook College, Seoul 139-742, Korea; 2 Laboratory
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Corresponding author:
Nam-Joo Ha , Tel: 82-2-3399-3653,
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Abstract
To determine the occurrence of vancomycin-resistant Enterococci in a raw milk sample, raw milk samples were examined for a period of 6 months. Enterococci were isolated directly from Enterococcal selective agar plates supplemented with 2 mg of vancomycin per liter. Nineteen strains were selected and identified by applying the Vitek system. To determine resistance patterns, 19 isolates were tested with vancomycin and teicoplanin. Vancomycin-resistant Enterococci were genotyped by using a PCR analysis and 5 out of 19 isolates were of the VanC type.
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