Characterization of the Proteolytic Activity of Bacteria Isolated from a Rotating Biological Contactor

In Jae Park; Jerng Chang Yoon; Seong Joo Park; Eung Ho Kim; Yeon-Jae Cho; Kwang-Soo Shin


Characterization of the Proteolytic Activity of Bacteria Isolated from a Rotating Biological Contactor: In Jae Park ¹, Jerng Chang Yoon ¹, Seong Joo Park ¹, Eung Ho Kim ², Yeon-Jae Cho ³, Kwang-Soo Shin ¹,; Journal of Microbiology 2003;41(2):73-77

1 Department of Microbiology, College of Sciences, Daejeon University, Daejeon 300-716, Korea; 2 Major in Civil Engineering, School of Urban & Civil Engineering, College of Engineering, Hong-Ik University, Seoul 121-791, Kore; 3 Seil Technology Co., LTD., Seoul 150-800, Korea (Received January 22, 2003 / Accepted March 22, 2003)1 Department of Microbiology, College of Sciences, Daejeon University, Daejeon 300-716, Korea; 2 Major in Civil Engineering, School of Urban & Civil Engineering, College of Engineering, Hong-Ik University, Seoul 121-791, Kore; 3 Seil Technology Co., LTD., Seoul 150-800, Korea (Received January 22, 2003 / Accepted March 22, 2003)

Corresponding author: , Tel: 82-42-280-2439,

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Abstract

Four proteolytic bacteria were isolated and identified from a rotating biological contactor based on Bacillus. The four isolates, Ni 26, 36, 39 and 49 were identified as B. vallismortis, B. subtilis, Aeromonas hydrophila and B. amyloliquefaciens, respectively, based on their biochemical properties and 16S rDNA sequence analyses. The optimal proteolytic activity was observed in the temperature and pH ranges of 40-70ºC and 8.0-8.5, respectively. The proteolytic activities of all the isolates were partially inhibited by phenylmethylsulfonylfluoride (PMSF), and the isolates Ni 26, Ni 39 and Ni 49 were inhibited by the metalloprotease inhibitor, 1,10-phenanthroline. Zymographic analyses of the culture supernatants revealed the presence of at least two proteases in all isolates.

Keywords: Aeromonas; Bacillus; proteolytic bacteria; rotating biological contactor

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Characterization of the Proteolytic Activity of Bacteria Isolated from a Rotating Biological Contactor

J. Microbiol. 2003;41(2):73-77.

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