Structural Analysis of the fcbABC Gene Cluster Responsible for Hydrolytic Dechlorination of 4-Chlorobenzoate from pJS1 Plasmid of Comamonas sp. P08

Jeong-Soon Lee; Kyoung Lee; Jong-Ok Ka; Jong-Chan Chae; Chi-Kyung Kim

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Structural Analysis of the fcbABC Gene Cluster Responsible for Hydrolytic Dechlorination of 4-Chlorobenzoate from pJS1 Plasmid of Comamonas sp. P08: Jeong-Soon Lee ¹, Kyoung Lee ², Jong-Ok Ka ³, Jong-Chan Chae ⁴, Chi-Kyung Kim ¹; Journal of Microbiology 2003;41(2):89-94

1 Department of Microbiology and Biotechnology, Chungbuk National University, Cheongju 361-763, Korea; 2 Department of Microbiology, Changwon National University, Changwon 641-773, Korea; 3 Department of Agricultural Biology, Seoul National University, Suwon 441-744, Korea; 4 Biotechnology Center for Agriculture and Environment, Rutgers University, New Brunswick, New Jersey 08901, USA (Received February 14, 2003 / Accepted March 13, 2003)1 Department of Microbiology and Biotechnology, Chungbuk National University, Cheongju 361-763, Korea; 2 Department of Microbiology, Changwon National University, Changwon 641-773, Korea; 3 Department of Agricultural Biology, Seoul National University, Suwon 441-744, Korea; 4 Biotechnology Center for Agriculture and Environment, Rutgers University, New Brunswick, New Jersey 08901, USA (Received February 14, 2003 / Accepted March 13, 2003)

Corresponding author: Chi-Kyung Kim , Tel: 82-43-261-2300,

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Abstract

Bacterial strain No. P08 isolated from wastewater at the Cheongju industrial complex was found to be capable of degrading 4-chlorobenzoate under aerobic condition. P08 was identified as Comamonas sp. from its cellular fatty acid composition and 16S rDNA sequence. The fcb genes, responsible for the hydrolytic dechlorination of 4-chlorobenzoate, were cloned from the plasmid pJS1 of Comamonas sp. P08. The fcb gene cluster of comamonas sp. P08 was organized in the order fcbB-fcbA-fcbT1-fcbT2-fcbT3-fcbC. This organization of the fcb genes was very similar to that of the fcb genes carried on the chromosomal DNA of Pseudomonas sp. DJ-12. However, it differed from the fcbA-fcbB-fcbC ordering of Arthrobacter sp. SU. The nucleotide sequences of the fcbABC genes of strain P08 showed 98% and 53% identities to those of Pseudomonas sp. DJ-12 and Arthrobacter sp. SU, respectively. This suggests that the fcb genes might have been derived from Pseudomonas sp. DJ-12 to form plasmid pJS1 in Comamonas sp. P08, or that the fcb genes in strain DJ-12 were transposed from Comamonas sp. P08 plasmid.

Keywords: fcb genes; pJS1 plasmid; hydrolytic dechlorination; 4-chlorobenzoate; Comamonas sp. P08

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Structural Analysis of the fcbABC Gene Cluster Responsible for Hydrolytic Dechlorination of 4-Chlorobenzoate from pJS1 Plasmid of Comamonas sp. P08

J. Microbiol. 2003;41(2):89-94.

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