Cloning and Sequence Analysis of Two Catechol-degrading Gene Clusters from a Phenol-utilizing Bacterium Pseudomonas putida SM25

Young-Hee Jung; Jong-Ok Ka; Choong-Ill Cheon; Myeong-Sok Lee; Eun-Sook Song; Soon-Young Choi; Daeho Cho; Sang-Ho Choi; Kwon-Soo Ha; Young Mok Park; Jong-Soon Choi; Kyung-Hee Min

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Cloning and Sequence Analysis of Two Catechol-degrading Gene Clusters from a Phenol-utilizing Bacterium Pseudomonas putida SM25: Young-Hee Jung ¹, Jong-Ok Ka ², Choong-Ill Cheon ¹, Myeong-Sok Lee ¹, Eun-Sook Song ¹, Soon-Young Choi ¹, Daeho Cho ¹, Sang-Ho Choi ³, Kwon-Soo Ha ⁴, Young Mok Park ⁵, Jong-Soon Choi ⁵, Kyung-Hee Min ¹; Journal of Microbiology 2003;41(2):102-108

1 Department of Biological Science, Sookmyung Women's University, Yongsan-ku_ Seoul 140-742, Korea; 2 Department of Agricultural Biology, Seoul National University, Suwon 441-744, Korea; 3 Department of Food Science & Technology, Chunnam National University, Gwangju 500-757, Korea; 4 Department of Molecular Cellular Biochemistry, Kangwon National University School of Medicine, Kangwon-do, 200-701; 5 Korea Basic Science Institute, Daejeon 305-333, Korea (Received February 10, 2003 / Accepted April 1, 2003)1 Department of Biological Science, Sookmyung Women's University, Yongsan-ku_ Seoul 140-742, Korea; 2 Department of Agricultural Biology, Seoul National University, Suwon 441-744, Korea; 3 Department of Food Science & Technology, Chunnam National University, Gwangju 500-757, Korea; 4 Department of Molecular Cellular Biochemistry, Kangwon National University School of Medicine, Kangwon-do, 200-701; 5 Korea Basic Science Institute, Daejeon 305-333, Korea (Received February 10, 2003 / Accepted April 1, 2003)

Corresponding author: Kyung-Hee Min , Tel: 82-2-710-9415,

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Abstract

A 6.1 kb Sph I fragment from the genomic DNA of Pseudomonas putida SM 25 was cloned into the vector pUC19. The open reading frame of catB was found to consist of 1,122 nucleotides. The sequence alignment of the catB gene products from different kinds of bacteria revealed an overall identity ranging from 40 to 98%. The catC gene contained an open reading frame of 96 codons, from which a protein with a molecular mass of about 10.6 kDa was predicted. The amino acids in the proposed activesite region of CatC were found to be almost conserved, including the charged residues. Since the catBC genes in P. putida SM25 were tightly linked, they could be regulated under coordinate transcription, and transcribed from a single promoter located upstream of the catB gene, as in P. putida RB1.

Keywords: Catechol degradation; catB and catC genes; cloning and sequence analysis

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Cloning and Sequence Analysis of Two Catechol-degrading Gene Clusters from a Phenol-utilizing Bacterium Pseudomonas putida SM25

J. Microbiol. 2003;41(2):102-108.

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