Isolation of Listeria monocytogenes by Immunomagnetic Separation and Atomic Force Microscopy

Birce Mercanoglu; S. Aykut Aytac; M. Ali Ergun; Erdal Tan

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Isolation of Listeria monocytogenes by Immunomagnetic Separation and Atomic Force Microscopy: Birce Mercanoglu ¹, S. Aykut Aytac ¹, M. Ali Ergun ², Erdal Tan ³; Journal of Microbiology 2003;41(2):144-147

1 Hacettepe University, Faculty of Engineering, Food Engineering Department, 06532, Beytepe, Ankara, Turke; 2 Gazi University, Faculty of Medicine, Department of Medical Biology and Genetics, 06510, Besevler, Ankara, Turkey; 3 Ankara Nuclear Research and Training Center, Material Research Department, 06105, Besevler, Ankara, Turkey (Received February 17, 2003 / Accepted May 9, 2003)1 Hacettepe University, Faculty of Engineering, Food Engineering Department, 06532, Beytepe, Ankara, Turke; 2 Gazi University, Faculty of Medicine, Department of Medical Biology and Genetics, 06510, Besevler, Ankara, Turkey; 3 Ankara Nuclear Research and Training Center, Material Research Department, 06105, Besevler, Ankara, Turkey (Received February 17, 2003 / Accepted May 9, 2003)

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Abstract

Listeria monocytogenes is a pathogen of major concern to the food industry and the potential cause of severe infections such as listeriosis. Early detection of this foodborne pathogen is important in order to eliminate its potential hazards. So, immunomagnetic separation (IMS) has been suggested as a means of reducing the total analysis time and for improving the sensitivity of detection. Atomic force microscopy (AFM) has been used for measuring the topographic properties of sample surfaces at nanometer scale. In this study, we used AFM to confirm both the sensitivity and the specificity of IMS. Regarding AFM analysis, the length and the width of the bacteria, which were in agreement with literature values, were found to be 2.993 mm and 0.837 mm, respectively. As a result, AFM helped us both characterize and measure the bacterial and bead structures.

Keywords: Listeria monocytogenes; atomic force microscopy; immunomagnetic separation

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Isolation of Listeria monocytogenes by Immunomagnetic Separation and Atomic Force Microscopy

J. Microbiol. 2003;41(2):144-147.

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