Use of Sucrose-Agar Globule with Root Exudates for Mass Production of Vesicular Arbuscular Mycorrhizal Fungi

Thangaswamy Selvaraj; Hoon Kim

doi:1998 [pii]

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Use of Sucrose-Agar Globule with Root Exudates for Mass Production of Vesicular Arbuscular Mycorrhizal Fungi: Thangaswamy Selvaraj ¹, ², Hoon Kim ²; Journal of Microbiology 2004;42(1):60-63
DOI: https://doi.org/1998 [pii]

1 Centre for Advanced Studies in Botany, University of Madras, Chennai 600-025, Tamil Nadu, INDIA; 2 Department of Agricultural Chemistry, and Research Institute of Agricultural Sciences, Sunchon National University, Suncheon 540-742, Korea.1 Centre for Advanced Studies in Botany, University of Madras, Chennai 600-025, Tamil Nadu, INDIA; 2 Department of Agricultural Chemistry, and Research Institute of Agricultural Sciences, Sunchon National University, Suncheon 540-742, Korea.

Corresponding author: Hoon Kim , Tel: 82-61-750-3294,

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Abstract

A sucrose-agar globule (SAG) was newly introduced to increase production of the vesicular arbuscular mycorrhizal (VAM) fungal spores, Gigaspora gigantea and Glomus fasciculatum. An SAG inoculum and a sucrose-agar globule with root exudates (SAGE) inoculum were prepared, and their spore productions were compared with a soil inoculum. When the SAGE was used as the inoculum on sucroseagar medium plates the number of spores was increased (35% more than the soil inoculum). After the soil inoculum and SAGE were inoculated on an experimental plant, Zingiber officinale, the percentage root colonization, number of VAM spores, and dry matter content were analyzed. It was observed that the SAGE showed a higher percentage of root colonization (about 10% more), and increases in the number of spores (about 26%) and dry matter (more than 13%) for the two VAM fungal spores than the soil inoculum. The results of this study suggested that the SAGE inoculum may be useful for the mass production of VAM fungi and also for the large scale production of VAM fungal fertilizer.

Keywords: VAM fungi; sucrose-agar globule inoculum; root exudates

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Use of Sucrose-Agar Globule with Root Exudates for Mass Production of Vesicular Arbuscular Mycorrhizal Fungi

J. Microbiol. 2004;42(1):60-63.

DOI: https://doi.org/1998 [pii]

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