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Research Support, Non-U.S. Gov't
- Optimal Fermentation Conditions for Enhanced Glutathione Production by Saccharomyces cerevisiae FF-8
- Jae-Young Cha 1, Jin-Chul Park 1, Beong-Sam Jeon 2, Young-Choon Lee 1, Young-Su Cho 1
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Journal of Microbiology 2004;42(1):51-55
DOI: https://doi.org/2000 [pii]
1 Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Korea; 2 BioHub Co., Ltd, Institute of Life Science (33)-617, Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea1 Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Korea; 2 BioHub Co., Ltd, Institute of Life Science (33)-617, Gyeongsang National University, Jinju, Gyeongnam 660-701, Korea
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Corresponding author:
Young-Su Cho , Tel: 82-51-200-7586,
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Abstract
The influence of feedstock amino acids, salt, carbon and nitrogen sources on glutathione production by Saccharomyces cerevisiae FF-8 was investigated. Glucose, yeast extract, KH_2PO_4, and L-cysteine were found to be suitable feedstock. Highest glutathione production was obtained after cultivation with shaking for 72 h in a medium containing glucose 3.0% (w/v), yeast extract 3.0%, KH_2PO_4 0.06% and L-cysteine 0.06%. The glutathione concentration achieved using this medium increased 2.27-fold to 204 mg/l compared to YM basal medium.
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