Molecular Cloning and Identification of a Novel Oxygenase Gene Specifically Induced during the Growth of Rhodococcus sp. Strain T104 on Limonene

Ki Young Choi; Dockyu Kim; Sung-Cheol Koh; Jae-Seong So; Jong-Sul Kim; Eungbin Kim

doi:2027 [pii]

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Molecular Cloning and Identification of a Novel Oxygenase Gene Specifically Induced during the Growth of Rhodococcus sp. Strain T104 on Limonene: Ki Young Choi ¹, Dockyu Kim ¹, Sung-Cheol Koh ², Jae-Seong So ³, Jong-Sul Kim ⁴, Eungbin Kim ¹; Journal of Microbiology 2004;42(2):160-162
DOI: https://doi.org/2027 [pii]

1 Laboratory of Environmental Microbial Genomics, Department of Biology, Yonsei University, Seoul 120-749, Korea; 2 Division of Civil and Environmental Engineering, Korea Maritime University, Pusan 606-791, Korea; 3 Department of Biological Engineering, Center of Advanced Bioseparation Technology, Inha University, Incheon 402-751, Korea; 4 Division of Life Science, University of Ulsan, Ulsan 680-749, Korea1 Laboratory of Environmental Microbial Genomics, Department of Biology, Yonsei University, Seoul 120-749, Korea; 2 Division of Civil and Environmental Engineering, Korea Maritime University, Pusan 606-791, Korea; 3 Department of Biological Engineering, Center of Advanced Bioseparation Technology, Inha University, Incheon 402-751, Korea; 4 Division of Life Science, University of Ulsan, Ulsan 680-749, Korea

Corresponding author: Eungbin Kim , Tel: 82-2-2123-2651,

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Abstract

Rhodococcus sp. strain T104 is able to utilize both limonene and biphenyl as growth substrates.Furthermore, T104 possesses separate pathways for the degradation of limonene and biphenyl. Previously, we found that a gene(s) involved in limonene degradation was also related to indigo-producing ability. To further corroborate this observation, we have cloned and sequenced a 8,842-bp genomic DNA region with four open reading frames, including one for indole oxygenase, which converts indole to indigo (a blue pigment). The reverse transcription PCR data demonstrated that the identified indole oxygenase gene is specifically induced by limonene, thereby implicating this gene in the degradation of limonene by T104.

Keywords: : Rhodococcus; limonene; biphenyl; indole oxygenase

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Molecular Cloning and Identification of a Novel Oxygenase Gene Specifically Induced during the Growth of Rhodococcus sp. Strain T104 on Limonene

J. Microbiol. 2004;42(2):160-162.

DOI: https://doi.org/2027 [pii]

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