Abstract

The interrelationship of expression of the two adjacent genes, HSP82 and CIN2 in Saccharomyces cerevisiae was investigated. The two genes are very close each other, such that the poly(A) addition site of the HSP82 gene is likely to be immediately followed by the promoter region of the CIN2 gene. Thus, transcription of the upstream HSP82 gene might affect expression of the CIN2 gene via promoter occlusion. Based on HSP82 and CIN2 mRNA analysis of the wild type, disruption mutant of the HAP82 gene, and conditional RNA polymerase II mutant strains, I conclude that CIN2 transcription is not affected by HSP82 transcription. However, surprisingly the level of CIN2 gene transcripts, but not HSP82, is heightened by cycloheximide treatment, presumably due to an increase in its stability. No change is observed in kinetics of HSP82 RNA induction by cycloheximide treatment.

• Keywords: Saccharomyces cerevisiae; CIN2; cycloheximide; heat shock; RNA analysis