Molecular Taxonomy of a Soil Actinomycete Isolate, KCCM10454 Showing Neuroprotective Activity by 16S rRNA and rpoB Gene Analysis

Bong-Hee Lee; Hong Kim; Hyun-Ju Kim; Yoon-Kyu Lim; Kyung-Hee Byun; Brian Hutchinson; Chang-Jin Kim; Young-Hwan Ko; Keun-Hwa Lee; Chang-Yong Cha; Yoon-Hoh Kook; Bum-Joon Kim

doi:2158 [pii]

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Molecular Taxonomy of a Soil Actinomycete Isolate, KCCM10454 Showing Neuroprotective Activity by 16S rRNA and rpoB Gene Analysis: Bong-Hee Lee ², Hong Kim ¹, Hyun-Ju Kim ¹, Yoon-Kyu Lim ³, Kyung-Hee Byun ², Brian Hutchinson ², Chang-Jin Kim ⁴, Young-Hwan Ko ⁵, Keun-Hwa Lee ⁶, Chang-Yong Cha ¹, Yoon-Hoh Kook ¹, Bum-Joon Kim ¹; Journal of Microbiology 2005;43(2):213-218
DOI: https://doi.org/2158 [pii]

¹Department of Microbiology, and Liver Research Institute, College of medicine, Seoul National University, Seoul 110-799, Republic of Korea, ²Department of Anatomy & Neurobiology,College of Medicine, Institute of Medical Science, Cheju National University, Jeju 690-756, Republic of Korea, ³Department of Veterinary Public Health, College of Veterinary Medicine, Cheju National University, Jeju 690-756, Republic of Korea, ⁴Korea Research Institute of Bioscience and Biotechnology, Yusung, Taejon 305-600, Republic of Korea, ⁵Department of Food Science and Engineering, Cheju National University, Jeju 690-756, Republic of Korea, ⁶Department of Microbiology, College of Medicine, Institute of Medical Science, Cheju National University, Jeju 690-756, Republic of Korea¹Department of Microbiology, and Liver Research Institute, College of medicine, Seoul National University, Seoul 110-799, Republic of Korea, ²Department of Anatomy & Neurobiology,College of Medicine, Institute of Medical Science, Cheju National University, Jeju 690-756, Republic of Korea, ³Department of Veterinary Public Health, College of Veterinary Medicine, Cheju National University, Jeju 690-756, Republic of Korea, ⁴Korea Research Institute of Bioscience and Biotechnology, Yusung, Taejon 305-600, Republic of Korea, ⁵Department of Food Science and Engineering, Cheju National University, Jeju 690-756, Republic of Korea, ⁶Department of Microbiology, College of Medicine, Institute of Medical Science, Cheju National University, Jeju 690-756, Republic of Korea

Corresponding author: Bum-Joon Kim , Tel: 82-2-740-8316,

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Abstract

Epilepsy constitutes a significant public health problem, and even the newest drugs and neurosurgical techniques have proven unable to cure the disease. In order to select a group of isolates which could generate an active compound with neuroprotective or antiepileptic properties, we isolated 517 actinomycete strains from soil samples taken from Jeju Island, in South Korea. We then screened these strains for possible anti-apoptotic effects against serum deprivation-induced hippocampal cell death, using the 3-(4, 5-dimethylthiazol-2-yl)2,5-diphenyl-tetrazolium bromide (MTT) assay as an in vitro test. The excitotoxic glutamate analog, kainic acid (KA), was used to induce seizures in experimental mice in our in vivo tests. As a result of this testing, we located one strain which exhibited profound neuroprotective activity. This strain was identified as a Streptomyces species, and exhibited the rifampin-resistant genotype, Asn(AAC)^442, according to the results of 16S rRNA and rpoB gene analyses

Keywords: epilepsy; antiepileptic activity; hippocampal cell; apoptosis; Streptomyces; rifampin resistant genotype

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Molecular Taxonomy of a Soil Actinomycete Isolate, KCCM10454 Showing Neuroprotective Activity by 16S rRNA and rpoB Gene Analysis

J. Microbiol. 2005;43(2):213-218.

DOI: https://doi.org/2158 [pii]

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