Identification of Non-mutans Streptococci Organisms in Dental Plaques Recovering on Mitis-Salivarius Bacitracin Agar Medium

So Young Yoo; Pyung Sik Kim; Ho-Keel Hwang; Seong-Hoon Lim; Kwang-Won Kim; Son-Jin Choe; Byung-Moo Min; Joong-Ki Kook

doi:2160 [pii]

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Identification of Non-mutans Streptococci Organisms in Dental Plaques Recovering on Mitis-Salivarius Bacitracin Agar Medium: So Young Yoo ¹, Pyung Sik Kim ², Ho-Keel Hwang ², Seong-Hoon Lim ³, Kwang-Won Kim ³, Son-Jin Choe ⁴, Byung-Moo Min ⁵, Joong-Ki Kook ¹; Journal of Microbiology 2005;43(2):204-208
DOI: https://doi.org/2160 [pii]

¹Department of Oral Biochemistry, College of Dentistry, Chosun University, 375 Seo-Suk Dong, Dong-Ku, Gwang-ju 501-759, Republic of Korea , ²Department of Conservative Dentistry, College of Dentistry, Chosun University, 375 Seo-Suk Dong, Dong-Ku, Gwang-ju 501-759, Republic of Korea, ³Department of Orthodontics, College of Dentistry, Chosun University, 375 Seo-Suk Dong, Dong-Ku, Gwang-ju 501-759, Republic of Korea, ⁴Department of Oral Microbiology and Immunology, College of Dentistry, Seoul National University, 28 Yeonkun-Dong, Chongno-Ku, Seoul 110-749, Republic of Korea , ⁵Department of Oral Biochemistry, College of Dentistry, Seoul National University, 28 Yeonkun-Dong, Chongno-Ku, Seoul 110-749, Republic of Korea¹Department of Oral Biochemistry, College of Dentistry, Chosun University, 375 Seo-Suk Dong, Dong-Ku, Gwang-ju 501-759, Republic of Korea , ²Department of Conservative Dentistry, College of Dentistry, Chosun University, 375 Seo-Suk Dong, Dong-Ku, Gwang-ju 501-759, Republic of Korea, ³Department of Orthodontics, College of Dentistry, Chosun University, 375 Seo-Suk Dong, Dong-Ku, Gwang-ju 501-759, Republic of Korea, ⁴Department of Oral Microbiology and Immunology, College of Dentistry, Seoul National University, 28 Yeonkun-Dong, Chongno-Ku, Seoul 110-749, Republic of Korea , ⁵Department of Oral Biochemistry, College of Dentistry, Seoul National University, 28 Yeonkun-Dong, Chongno-Ku, Seoul 110-749, Republic of Korea

Corresponding author: Joong-Ki Kook , Tel: 82-62-230-6877,

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Abstract

The objective of this study was to both isolate and identify non-mutans streptococci organisms (non-MSO) from dental plaques recovered on mitis-salivarius sucrose bacitracin agar (MSB) plates. The dental plaque samples, which had been collected from 63 human subjects, were diluted and plated on MSB. The bacteria growing on the MSB plates were then identified with biochemical tests, as well as with 16S rDNA cloning and sequencing techniques. Our data indicated that bacteria from 30 subjects had been recovered on the MSB plates. Among the 21 typical colonies selected from the 30 subjects, 12 colonies, derived from 10 subjects, were identified as non-MSO. These 12 colonies were determined to be Streptococcus anginosus (8 colonies), S. sanguinis (1 colony), and Pantoea agglomerans (3 colonies). These results strongly suggest that a new selective medium will be required for the reliable isolation of mutans streptococci.

Keywords: dental plaque; mitis-salivarius bacitracin agar; non-mutans streptococci organism; 16S rDNA

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Identification of Non-mutans Streptococci Organisms in Dental Plaques Recovering on Mitis-Salivarius Bacitracin Agar Medium

J. Microbiol. 2005;43(2):204-208.

DOI: https://doi.org/2160 [pii]

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