Abstract

Strain Kw07^T, a Gram-negative, non-spore-forming, rod-shaped bacterium, was isolated from granules in an Up-flow Anaerobic Sludge Blanket (UASB) bioreactor used in the treatment of brewery wastewater. 16S rRNA gene sequence analysis revealed that strain Kw07^T belongs to the [alpha]-4 subclass of the Proteobacteria, and the highest degree of sequence similarity was determined to be to Sphingopyxis macrogoltabida IFO 15033^T (97.8%). Chemotaxonomic data revealed that strain Kw07^T possesses a quinone system with the predominant compound Q-10, the predominant fatty acid C_18:1 w7c, and sphingolipids, all of which corroborated our assignment of the strain to the Sphingopyxis genus. The results of DNA-DNA hybridization and physiological and biochemical tests clearly demonstrated that strain Kw07^T represents a distinct species. Based on these data, Kw07^T (= KCTC 12209^T = NBRC 100800^T) should be classified as the type strain for a novel Sphingopyxis species, for which the name Sphingopyxis granuli sp. nov. has been proposed.

• Keywords: [alpha]-Proteobacteria; Sphingomonadaceae; Sphingopyxis granuli