Characterization of the 5'-Flanking region upstream from the structural Gene for Zymomonas mobilis Alcohol Dehydrogenase

Yoon, Ki Hong; Park, Seung Hwan; Jung, Kyung Hwa; Pack, M. Y.


Characterization of the 5'-Flanking region upstream from the structural Gene for Zymomonas mobilis Alcohol Dehydrogenase: Yoon, Ki Hong , Park, Seung Hwan , Jung, Kyung Hwa , Pack, M. Y. ¹; Journal of Microbiology 1995;33(2):126-127

Korea Research Institute of Bioscience and Biotechnology, KIST; ¹Department of Biological Science KAISTKorea Research Institute of Bioscience and Biotechnology, KIST; ¹Department of Biological Science KAIST

Corresponding author: Yoon, Ki Hong ,

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Abstract

A Zymomonas mobilis DNA fragment consisting of 207 nucleotides, which corresponded to the 5'-flanking region of an adhB gene encoding alcohol dehydrogenase II, was fused to the structural gene coding for a Bacillus endo-β-1, 4-glucanase. The Z. mobilis DNA fragment was identified to promote 50-fold increase in the expression of endo-β-1,4 glucanase gene in Escherichia coli.

Keywords: Zymomonas mobilis; promoter alcohol dehydrogenase; endo-β-1;4-glucanase

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Characterization of the 5'-Flanking region upstream from the structural Gene for Zymomonas mobilis Alcohol Dehydrogenase

J. Microbiol. 1995;33(2):126-127.

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