Development of Strain-specific PCR Primers Based on a DNA Probe Fu12 for the Identification of Fusobacterium nucleatum subsp. nucleatum ATCC 25586^T

Hwa-Sook Kim; Soo Keun Song; So Young Yoo; Dong Chun Jin; Hwan Seon Shin; Chae Kwang Lim; Myung-Soo Kim; Jin-Soo Kim; Son-Jin Choe; Joong-Ki Kook

doi:2257 [pii]

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Research Support, Non-U.S. Gov't
Development of Strain-specific PCR Primers Based on a DNA Probe Fu12 for the Identification of Fusobacterium nucleatum subsp. nucleatum ATCC 25586^T: Hwa-Sook Kim ¹, Soo Keun Song ¹, So Young Yoo ¹, Dong Chun Jin ², Hwan Seon Shin ¹, Chae Kwang Lim ¹, Myung-Soo Kim ², Jin-Soo Kim ³, Son-Jin Choe ⁴, Joong-Ki Kook ¹; Journal of Microbiology 2005;43(4):331-336
DOI: https://doi.org/2257 [pii]

¹Department of Oral Biochemistry, College of Dentistry, Chosun University, 375 Seo-Suk Dong, Dong-ku, Gwang-ju 501-759, Republic of Korea, ²Department of Dental Pharmacology, College of Dentistry, Chosun University, 375 Seo-Suk Dong, Dong-ku, Gwang-ju 501-759, Republic of Korea, ³Department of Oral and Maxillofacial Radiology, College of Dentistry, Chosun University, 375 Seo-Suk Dong, Dong-ku, Gwang-ju 501-759, Republic of Korea, ⁴Department of Oral Microbiology and Immunology, College of Dentistry, Seoul National University, 28 Yeonkun-Dong, Chongno-Ku, Seoul 110-749, Republic of Korea¹Department of Oral Biochemistry, College of Dentistry, Chosun University, 375 Seo-Suk Dong, Dong-ku, Gwang-ju 501-759, Republic of Korea, ²Department of Dental Pharmacology, College of Dentistry, Chosun University, 375 Seo-Suk Dong, Dong-ku, Gwang-ju 501-759, Republic of Korea, ³Department of Oral and Maxillofacial Radiology, College of Dentistry, Chosun University, 375 Seo-Suk Dong, Dong-ku, Gwang-ju 501-759, Republic of Korea, ⁴Department of Oral Microbiology and Immunology, College of Dentistry, Seoul National University, 28 Yeonkun-Dong, Chongno-Ku, Seoul 110-749, Republic of Korea

Corresponding author: Joong-Ki Kook , Tel: 82-62-230-6877,

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Abstract

The objective of this study was to assess the strain-specificity of a DNA probe, Fu12, for Fusobacterium nucleatum subsp. nucleatum ATCC 25586^T (F. nucleatum ATCC 25586^T), and to develop sets of strain-specific polymerase chain reaction (PCR) primers. Strain-specificity was tested against 16 strains of F. nucleatum and 3 strains of distinct Fusobacterium species. Southern blot hybridization revealed that the Fu12 reacted exclusively with the HindIII-digested genomic DNA of F. nucleatum ATCC 25586^T. The results of PCR revealed that three pairs of PCR primers, based on the nucleotide sequence of Fu12, generated the strain-specific amplicons from F. nucleatum ATCC 25586^T. These results suggest that the DNA probe Fu12 and the three pairs of PCR primers could be useful in the identification of F. nucleatum ATCC 25586^T, especially with regard to the determination of the authenticity of the strain.

Keywords: DNA probe Fu12; identification; Fusobacterium nucleatum ATCC 25586^T; strain-specific PCR primers

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Development of Strain-specific PCR Primers Based on a DNA Probe Fu12 for the Identification of Fusobacterium nucleatum subsp. nucleatum ATCC 25586^T

J. Microbiol. 2005;43(4):331-336.

DOI: https://doi.org/2257 [pii]

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