Immunization with Major Outer Membrane Protein of Vibrio vulnificus Elicits Protective Antibodies in a Murine Model

Cho-Rok Jung; Min-Jung Park; Moon-Soo Heo

doi:2278 [pii]

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Immunization with Major Outer Membrane Protein of Vibrio vulnificus Elicits Protective Antibodies in a Murine Model: Cho-Rok Jung ¹, Min-Jung Park ², Moon-Soo Heo ³; Journal of Microbiology 2005;43(5):437-442
DOI: https://doi.org/2278 [pii]

¹Laboratory of Cellular Biology, Korea Research Institute of Bioscience, Biotechnology (KRIBB), Yusung, Daejon 305-333, Republic of Korea, ²Department of Quality Control of Herbal Medicine, Korea Institute of Oriental Medicine, Yusung, Taejon 461-24, Republic of Korea, ³Faculty of Marine Science, Cheju National University, Jeju 690-756, Republic of Korea¹Laboratory of Cellular Biology, Korea Research Institute of Bioscience, Biotechnology (KRIBB), Yusung, Daejon 305-333, Republic of Korea, ²Department of Quality Control of Herbal Medicine, Korea Institute of Oriental Medicine, Yusung, Taejon 461-24, Republic of Korea, ³Faculty of Marine Science, Cheju National University, Jeju 690-756, Republic of Korea

Corresponding author: Moon-Soo Heo , Tel: 82-64-754-3473,

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Abstract

Sera from rabbits were infected with Vibrio vulnificus containing an antibody against major outer membrane protein (MOMP). MOMP of V. vulnificus ATCC 27562 were isolated and purified by Sarkosyl and TritonX-100 dual treatment. Molecular size of MOMP was identified as 36-kDa on 13% SDS-PAGE. The sequence of the first 26 amino acid residues from the N-terminal end of the protein is AELYNQDGTSLDMGGRAEARLSMKDG , which is a perfect match with OmpU of V. vulnificus CMCP6 and YJ016. MOMP specific IgM and IgG were investigated in groups of mice. The group of mice immunized with MOMP and Alum showed higher levels of IgG2b than the group immunized with only MOMP. Vaccination with MOMP resulted in protective antibodies in the mouse infection experiment.

Keywords: immunogenecity; outer membrane protein; vaccine; Vibrio vulnificus

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Immunization with Major Outer Membrane Protein of Vibrio vulnificus Elicits Protective Antibodies in a Murine Model

J. Microbiol. 2005;43(5):437-442.

DOI: https://doi.org/2278 [pii]

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