Production of Saccharogenic and Dextrinogenic Amylases by Rhizomucor pusillus A 13.36

Tony M. Silva; Derlene Attili-Angelis; Ana Flavia Azevedo Carvalho; Roberto Da Silva; Mauricio Boscolo; Eleni Gomes

doi:2289 [pii]

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Research Support, Non-U.S. Gov't
Production of Saccharogenic and Dextrinogenic Amylases by Rhizomucor pusillus A 13.36: Tony M. Silva ¹, Derlene Attili-Angelis ², Ana Flavia Azevedo Carvalho ¹, Roberto Da Silva ¹, Mauricio Boscolo ³, Eleni Gomes ¹; Journal of Microbiology 2005;43(6):561-568
DOI: https://doi.org/2289 [pii]

¹Laboratorio de Bioquimica e Microbiologia, IBILCE-Instituto de Instituto de Biociencias, Letras e Ciencias Exatas, UNESP- Universidade Estadual Paulista., Sao Jose do Rio Preto- SP, Brazil, ²Departamento de Bioquimica e Microbiologia, Instituto de Biociencias, UNESP-Universidade EstadualPaulista, Rio Claro-SP, Brazil, ³Laboratorio de Fisico-Quimica, IBILCE-Instituto de Instituto de Biociencias, Letras e Ciencias Exatas,UNESP-Universidade Estadual Paulista., Sao Jose do Rio Preto- SP, Brazil¹Laboratorio de Bioquimica e Microbiologia, IBILCE-Instituto de Instituto de Biociencias, Letras e Ciencias Exatas, UNESP- Universidade Estadual Paulista., Sao Jose do Rio Preto- SP, Brazil, ²Departamento de Bioquimica e Microbiologia, Instituto de Biociencias, UNESP-Universidade EstadualPaulista, Rio Claro-SP, Brazil, ³Laboratorio de Fisico-Quimica, IBILCE-Instituto de Instituto de Biociencias, Letras e Ciencias Exatas,UNESP-Universidade Estadual Paulista., Sao Jose do Rio Preto- SP, Brazil

Corresponding author: Eleni Gomes , Tel: 55-017-2212393,

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Abstract

A newly-isolated thermophilic strain of the zygomycete fungus Rhizomucor pusillus 13.36 produced highly active dextrinogenic and saccharogenic enzymes. Cassava pulp was a good alternative substrate for amylase production. Dextrinogenic and saccharogenic amylases exhibited optimum activities at a pH of 4.0-4.5 and 5.0 respectively and at a temperature of 75oC. The enzymes were highly thermostable, with no detectable loss of saccharogenic or dextrinogenic activity after 1 h and 6 h at 60oC, respectively. The saccharogenic activity was inhibited by Ca2+ while the dextrinogenic was indifferent to this ion. Both activities were inhibited by Fe2+ and Cu2+ Hydrolysis of soluble starch by the crude enzyme yielded 66% glucose, 19.5% maltose, 7.7% maltotriose and 6.6% oligosaccharides.

Keywords: amylase; Rhizomucor pusillus; thermostability

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Production of Saccharogenic and Dextrinogenic Amylases by Rhizomucor pusillus A 13.36

J. Microbiol. 2005;43(6):561-568.

DOI: https://doi.org/2289 [pii]

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